Indoximod (1-Methyl-D-tryptophan) is an orally activeindoleamine 2,3-dioxygenase(IDO) pathway inhibitor. Indoximod acts as a Trp mimetic in regulatingmTOR. Indoximod is an immunometabolic adjuvant used for the research ofcancer^[1][2].

The IDO inhibitor 1-methyl-tryptophan exists in two stereoisomers with potentially different biological properties. The L isomer is the more potent inhibitor of IDO activity using the purified enzyme and in HeLa cell–based assays. However, the D isomer is significantly more effective in reversing the suppression of T cells created by IDO-expressing dendritic cells. The L isomer of 1-methyl-tryptophan functioned as a competitive inhibitor (K_i=19 μM), whereas the d isomer is much less effective. The DL mixture is intermediate, with a K_iof 35 μM^[1].

The D isomer is more efficacious as an anticancer agent in chemo-immunotherapy regimens using NSC-26271, NSC 125973, or LY 188011, when tested in mouse models of transplantable melanoma and transplantable and autochthonous breast cancer. The D isomer of 1-methyl-tryptophan specifically targets the IDO gene because the antitumor effect of d-1-methyl-tryptophan is completely lost in mice with a disruption of the IDO gene (IDO-knockout mice). Oral administration of dl-1-methyl-tryptophan in combination with NSC 125973 can elicit regression of autochthonous breast tumors^[1].

218.25

Solid

C₁₂H₁₄N₂O₂

110117-83-4

1-甲基-D-色氨酸

Room temperature in continental US; may vary elsewhere.

H₂O : 5 mg/mL(22.91 mM;ultrasonic and adjust pH to 2 with HCl)

DMSO : 0.55 mg/mL(2.52 mM;Need ultrasonic and warming)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 1 mg/mL (4.58 mM); Clear solution; Need ultrasonic and warming and heat to 60℃