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6-Diazo-5-oxo-L-nor-Leucine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
6-Diazo-5-oxo-L-nor-Leucine图片
CAS NO:157-03-9
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
1mg电议
5mg电议
10mg电议
50mg电议

产品名称
6-重氮-5-氧代-L-正亮氨酸
L-6-Diazo-5-oxonorleucine
DON
产品介绍
6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) 是一种谷氨酰胺拮抗剂,能不可逆地抑制谷氨酰胺的分解作用。6-Diazo-5-oxo-L-nor-Leucine 具有较好的抗癌活性 (尤其是胰腺癌),能降低肿瘤细胞的自我更新潜力和转移能力。6-Diazo-5-oxo-L-nor-Leucine 还具有抗菌和抗病毒活性。
生物活性

6-Diazo-5-oxo-L-nor-Leucine (L-6-Diazo-5-oxonorleucine; DON) is aglutamineantagonist that irreversibly inhibits the catabolic effect of glutamine. 6-Diazo-5-oxo-L-nor-Leucine shows good anticancer activity (especially in pancreatic cancer) and reduces the self-renewal potential and metastatic capacity of tumour cells. 6-Diazo-5-oxo-L-nor-Leucine also possesses antibacterial and antiviral activity[1][2][3].

IC50& Target

Bacterial[3].

体外研究
(In Vitro)

6-Diazo-5-oxo-L-nor-Leucine (DON) (0.3 mM; 1 h) shows inhibition of glutamine catabolism in WI-L2 cells[1].
6-Diazo-5-oxo-L-nor-Leucine (DON) decreases the selfrenewal potential and metastatic ability of tumor cell[2].

Cell Viability Assay[1]

Cell Line:WI-L2 cells
Concentration:0.3 mM
Incubation Time:1 h
Result:Inhibited the rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 greater than 95%.
体内研究
(In Vivo)

6-Diazo-5-oxo-L-nor-Leucine sensitizes pancreatic tumors to anti-PD1 resulting in tumor regression and prolonged survival in vivo[2].
6-Diazo-5-oxo-L-nor-Leucine decreases hyaluronan and collagen in the tumor microenvironment, leading to an extensive remodeling of the ECM (extensive extracellular matrix), and an increases infiltration CD8+T-cells[2].

分子量

171.15

性状

Solid

Formula

C6H9N3O3

CAS 号

157-03-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 25 mg/mL(146.07 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM5.8428 mL29.2141 mL58.4283 mL
5 mM1.1686 mL5.8428 mL11.6857 mL
10 mM0.5843 mL2.9214 mL5.8428 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。