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Piperine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Piperine图片
CAS NO:94-62-2
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
200mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
胡椒碱
Bioperine
1-piperoylpiperidine
产品介绍
Piperine是从胡椒中分离的天然生物碱,可以抑制P-glycoproteinandCYP3A4的活性,在HeLa细胞中的IC50值为61.94±0.054 μg/mL。
生物活性

Piperine, a natural alkaloid isolated fromPiper nigrumL, inhibitsP-glycoproteinandCYP3A4activities with anIC50value of 61.94±0.054 μg/mL in HeLa cell.

IC50& Target

IC50: 61.94±0.054 μg/mL (P-glycoprotein, HeLa cell)[1]

体外研究
(In Vitro)

Piperine has shown to possessin vitrocytotoxic activity and in silico studies. The IC50value is found to be 61.94±0.054 μg/mL and in silico studies, it has more number of hydrogen bonds with minimum binding and docking energy and may be considered as inhibitor of EGFR tyrosine kinase[1]. Piperine has been found to have immunomodulatory, anti-oxidant, anti-asthmatic, anti-carcinogenic, anti-inflammatory, anti-ulcer, and anti-amoebic properties[2]. Piperine could enhance the bioavailabilities of other drugs including rosuvastatin, peurarin and docetaxel (DOX) via inhibition of CYP3A and P-glycoprotein activity[3].

体内研究
(In Vivo)

At the dose of 3.5 mg/kg, the bioavailability of piperine is calculated to be 25.36%. Its AUC0→tis unproportionally increased with doses, indicating a potential non-linear pharmacokinetics profile of piperine. It is found that the AUC0→tand C0of docetaxel and t1/2of piperine are significantly increased after their combination use, suggesting potential enhanced bioavailability of not only docetaxel but also piperine, which may lead to the overall enhanced pharmacological effects[3]. The phosphorylation of I-κB, p65, p38, ERK, and JNK is inhibited by piperine in a dose-dependent manner, indicating that piperine may be a potential anti-inflammatory drug both in endometritis and in otherS. aureus-induced diseases[4].

Clinical Trial
分子量

285.34

性状

Solid

Formula

C17H19NO3

CAS 号

94-62-2

中文名称

胡椒碱;胡椒酰胺

结构分类
  • Alkaloids
  • Piperidine Alkaloids
来源
  • Plants
  • Piperaceae
  • Piper nigrumLinn.
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL(175.23 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.5046 mL17.5230 mL35.0459 mL
5 mM0.7009 mL3.5046 mL7.0092 mL
10 mM0.3505 mL1.7523 mL3.5046 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.76 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.76 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (8.76 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.76 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。