Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) 是双链 RNA 的合成类似物，是一种TLR3和视黄酸诱导型基因 I 受体 (RIG-I和MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。
| 生物活性 | Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) is a synthetic analog of double-stranded RNA and an agonist oftoll-like receptor 3(TLR3)andretinoic acid inducible gene I (RIG-I)-like receptors(RIG-IandMDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid sodium can directly triggercancercells to undergoapoptosis[1][2]. |
| IC50& Target | |
体外研究
(In Vitro) | Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours[1].
Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells[1].
Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1[1].
Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells[1].
Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 ℃ at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 ℃ until use[3].
Cell Viability Assay[1] | Cell Line: | WM793, WM278, WM239A, WM9 and 1205Lu cells | | Concentration: | 20 ng/mL | | Incubation Time: | 24 hours | | Result: | Strongly reduced viability from 100% in controls to 20%–50% within 24 hours. |
Apoptosis Analysis[1] | Cell Line: | 1205Lu cells | | Concentration: | 200 ng/mL | | Incubation Time: | 24 hours | | Result: | Induced apoptosis in 1205Lu cells. |
RT-PCR[1] | Cell Line: | 1205Lu cells | | Concentration: | 3 ng/mL | | Incubation Time: | 24 hours | | Result: | Induced IFN-β expression in melanoma cells. |
Western Blot Analysis[1] | Cell Line: | 1205Lu cells | | Concentration: | 5 ng/mL | | Incubation Time: | 24 hours | | Result: | Revealed active subunits of caspase-9 and caspase-8 in melanoma cells. |
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体内研究
(In Vivo) | Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid[1].
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| 性状 | |
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x.xNa |
| CAS 号 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| 溶解性数据 | In Vitro: H2O : 10 mg/mL(Need ultrasonic and warming) In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 6.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic and warming and heat to 60℃
*以上所有助溶剂都可在本网站选购。 |
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