| 包装 | 价格(元) |
| 5mg | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
Cell lines | H460 cells |
Preparation Method | Migration and invasion assays were performed using cells serum-starved overnight. For migration assays, H-460 cells were seeded in Transwell inserts in RPMI 1640 with or without drugs or Nigericin sodium salt(1 uM). Inserts were placed in plates with RPMI 1640 containing 10% FBS in the presence of DMSO, or Nigericin sodium salt (1 uM). After 24 h, cell migration was quantified. |
Reaction Conditions | 1 μM Nigericin sodium salt for 24h |
Applications | Nigericin sodium salt inhibited the migration and invasion of H460 lung cancer cells. |
Animal models | Nude mice(S18 cells were injected near the scapula of the nude mice) |
Preparation Method | The mice were randomly divided into four groups with six animals each. DDP (2.5 mg/kg) was injected intraperitoneally for five continuous days and Nigericin sodium salt (4 mg/kg) was administrated intraperitoneally every two days. |
Dosage form | 4 mg/kg Nigericin sodium salt every two days. |
Applications | Nigericin sodium salt significantly reduced tumor growth. |
| 文献引用 | |
| 产品描述 | Nigericin sodium salt is an electrically neutral K +/H + ionophore from Streptomyctus hygroscopicus an antibiotic that is lipophilic and selectively causes potassium to drain from the mitochondrial membrane[8,9]. Nigericin sodium salt is a NLRP3 activator[1]. Nigericin sodium salt inhibited the migration and invasion of H460 lung cancer cells[1]. Nigericin sodium salt (0.1-10 nM) has apparently a dual effect on cell volume, that is a shrinking effect at lower Nigericin sodium salt concentrations and a swelling effect at higher concentrations[2].Nigericin sodium salt exhibits higher toxicity on S18 cells than S26 cells, with IC50 of 2.03±0.55 μM and 4.77±2.35 μM, respectively. Nigericin sodium salt dramatically reduces the migration ability of S18 and HONE-1 cells[3]. Nigericin sodium salt exhibits toxicity for the HT29 and SW116 cell line with IC50 of 12.92±0.25 μmol and 15.86±0.18 μmol. Nigericin sodium salt also shows a decreased ability to form colonies under anchorage-independent conditions in a standard soft agar assay[4].Using a combination of live-cell imaging and biochemical approaches, prototypical NLRP3 stimuli, the potassium ionophore nigericin, or millimolar concentrations of ATP exert both NLRP3-independent and -dependent effects on macrophages that result in cytokine release and cell death[5].Treatment of MN9D cells with Nigericin sodium salt led to an increase of LC3-II and p62 levels with concomitant activation of caspase. Ultrastructural examination revealed accumulation of autophagic vacuoles and swollen vacuoles in nigericin-treated cells[7]. In mice, Ngericin significantly reduces tumor growth. Nigericin sodium salt markedly decreases Bmi-1 in vivo. Overexpression of Bmi-1 partially restores CSC content and metastatic ability of NPC cells under Nigericin sodium salt treatment. The downregulation of Bmi-1 may be involved in the inhibitory effect of Nigericin sodium salt on CSCs in NPC[3].Nigericin sodium salt exerts anticancer effects on human osteosarcoma cancer cells by directly targeting STAT3. Nigericin sodium salt can significantly inhibit tumor migration and invasion. Nigericin sodium salt inhibits tumor growth in a mouse osteosarcoma model[6]. References: |
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