| CAS NO: | 927866-58-8 |
| 包装: | 10mg |
| 市场价: | 6395元 |
| Cas No. | 927866-58-8 | ||
| 化学名 | 2-((1-(4-aminobutanoyl)-7-nitroindolin-4-yl)oxy)propane-1,3-diyl bis(dihydrogen phosphate) | ||
| Canonical SMILES | NCCCC(N1CCC2=C(OC(COP(O)(O)=O)COP(O)(O)=O)C=CC(N(=O)=O)=C21)=O | ||
| 分子式 | C15H23N3O12P2 | ||
| 分子量 | 499.3 | ||
| 溶解度 | <1mg> | 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request | ||
| 产品描述 | IC50: N/A DPNI-GABA, also known as Nitroindoline-caged GABA, has similar photochemical properties with MNI-glutamate, including the same quantum yield (0.085), highly water soluble, exhibitting fast photorelease that is efficient at near-UV and 405 nm wavelengths. In vitro: Under the condition of retaining the advantages of nitroindoline cages, DPNI-GABA were modified to minimize the pharmacological interference commonly found with caged GABA reagents. Unlike previous test of the 5-methoxycarbonylmethyl-7-nitroindolinyl-GABA, DPNI-GABA reduced peak GABA-evoked responses with an IC50 of approximately 0.5mM, and blocked GABAA receptors with much lower affinity,. It is most important that the kinetics of receptor activation, comparable to synaptic events, were determined as 10–90% rise-times, and had no effect by DPNI-GABA present at 1 mM concentration, and permitted photolysis of DPNI-GABA to mimic synaptic activation of GABAA receptors. As estimated, the spatial resolution of uncaging DPNI-GABA in dendrites was 2m laterally and 7.5m focally with a laser spot of 1mapplied to cerebellar molecular layer interneurons. Finally, photorelease restricted to the area of the soma inhibited spiking in single Purkinje neurons or molecular layer interneurons for periods regulated by the flash intensity and duration at low DPNI-GABA concentration [1,2]. Under resting conditions, miniature synaptic currents have long been known to represent random transmitter release, but their nature and function in central synapses much remains to be learned about. A new class of miniature currents (‘‘preminis’’) arise by the autocrine activation of axonal receptors following random vesicular release. During the development of the molecular layer, preminis are prominent in gabaergic synapses made by cerebellar interneurons. Premini frequencies, unlike ordinary miniature postsynaptic currents in the same cells, are strongly enhanced by subthreshold depolarization, suggesting that themembrane depolarization they produce belongs to a feedback loop controlling neurotransmitter release. Thus, preminis enhanced neurotransmitter release to guide the formation of the interneuron network at recently formed synaptic contacts [3]. In vivo: So far, no study in vivo has been conducted Clinical trial: So far, no clinical study has been conducted. References: | ||
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