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Trypsin
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
CAS NO:9002-07-7
包装与价格：
包装价格(元)
100mg电议
500mg电议
1 g电议
5 g电议
10 g电议

产品介绍
Trypsin 是一种丝氨酸蛋白酶，可水解赖氨酸或精氨酸羧基侧蛋白质。Trypsin 激活PAR2和PAR4。Trypsin 通过 PDCoV 的 S 糖蛋白与 pAPN 的相互作用诱导 PDCoV 感染的细胞膜融合。Trypsin 还促进细胞增殖和分化。Trypsin 可用于伤口愈合和神经源性炎症的研究。
生物活性

Trypsin is a serine protease enzyme, and hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activatesPAR2andPAR4. Trypsin induces cell-to-cell membrane fusion in PDCoVinfectionby the interaction of Sglycoproteinof PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

IC50& Target

PAR2, PAR4[6]

体外研究
(In Vitro)

Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2].
Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2].
Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3].
Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4].

Western Blot Analysis[2]

Cell Line:LLC-PK cells, ST cells
Concentration:5 μg/mL
Incubation Time:24 or 48 h
Result:Promoted PDCoV replication in LLC-PK cells but not ST cells.

Immunofluorescence[2]

Cell Line:LLC-PK cells, ST cells
Concentration:10 and 50 ng/mL
Incubation Time:12 h
Result:Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.
体内研究
(In Vivo)

Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5].

Animal Model:Swiss mice[5]
Dosage:100-500 μg per site, in saline (50 μL)
Administration:Intradermal injection
Result:Induced pruritus, and was inhibited by trypsin inhibitor.
Clinical Trial
性状

Solid

CAS 号

9002-07-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month