THX-B 是一种有效的、非多肽类型的p75NTR(神经营养蛋白受体 p75) 拮抗剂。THX-B 可用于糖尿病、肾病、神经退行性和炎症性疾病的研究。
| 生物活性 | THX-B is a potent and non-peptidicp75NTR(neurotrophin receptor p75) antagonist. THX-B can be used in the research of diabetic kidney disease, neurodegenerative and inflammatory disorders[1][2][3]. |
体外研究
(In Vitro) | THX-B (10 μM, 4 days) decreases proliferation of myoblasts[1].
THX-B (10 μM, 1 h) inhibits NGF-induced phosphorylation of ERK1/2 in C2C12 myoblasts[1].
THX-B (20 μM, 24 h) decreases photoreceptor cell death and reactive gliosis in cultured rd10 retinas[2].
Western Blot Analysis[1] | Cell Line: | C2C12 myoblasts | | Concentration: | 10 μM | | Incubation Time: | Pre-treated for 1 hour | | Result: | Inhibited βNGF-induced ERK2 phosphorylation by 67%.
Inhibited proNGF-induced ERK2 phosphorylation by 90%. |
Immunofluorescence[1] | Cell Line: | Cultured P22 rd10 retinas. | | Concentration: | 20 μM | | Incubation Time: | 24 h | | Result: | Attenuated the thickening and enlargement of processes of astrocytes and Müller glia cells. |
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体内研究
(In Vivo) | THX-B (50 μg in 125 μL PBS, i.p. weekly for 4 weeks) improves bladder function in a mouse model of diabetic voiding dysfunction[3].
THX-B (2 μL of 2 μg/μL, IVT injection, a single dose) elicits a neuroprotective effect on photoreceptor cells in P17 rd10 mice[2].
THX-B (40 μg in 20 μL, IVT injection) resolves the inflammatory, vascular, and neurodegenerative phases of the retinal pathology[4].
| Animal Model: | Mouse model of diabetic voiding dysfunction | | Dosage: | 50 μg in 125 μL PBS | | Administration: | Intraperitoneal injection (i.p.) | | Result: | Prevented bladder weight increase, which was 18% (95% CI 3%, 32%) and 37% (95% CI 14%, 60%) lower after 2 and 4 weeks of treatment. |
| Animal Model: | P17 rd10 mice[1] | | Dosage: | 2 μL of 2 μg/μL, single dose | | Administration: | Intravitreal (IVT) injected in one eye | | Result: | Increased the number of photoreceptor rows as well as the ONL/INL ratio.
Decreased the total number of microglial cells in the treated retinas, as well as some of the inflammatory signs, such as GFAP, α2M and the proinflammatory cytokines IL-1β and TNFα. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(274.42 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.7442 mL | 13.7212 mL | 27.4424 mL | | 5 mM | 0.5488 mL | 2.7442 mL | 5.4885 mL | | 10 mM | 0.2744 mL | 1.3721 mL | 2.7442 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
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