Doxycycline hydrochloride 是一种具有口服活性的四环素抗生素，是广谱的金属蛋白酶 (MMP) 抑制剂，具有抗菌活性和抗癌细胞增殖活性。
| 生物活性 | Doxycycline hydrochloride, anantibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline hydrochloride shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5]. |
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体外研究
(In Vitro) | Doxycycline hydrochloride (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
Doxycycline hydrochloride (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells[2].
Doxycycline hydrochloride (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines[4].
Doxycycline hydrochloride (0-250 μM, 72 h) inhibits cell viability of breast cancer cells[5].
Cell Viability Assay[2] | Cell Line: | LNT-229, G55, and U343 glioma cells | | Concentration: | 0.01, 0.1, 1 or 10 μg/mL | | Incubation Time: | 4 days | | Result: | Affected growth of glioma cells only under high concentrations (10 μg/mL). |
Cell Viability Assay[2] | Cell Line: | SVG cells | | Concentration: | 0.01, 0.1, 1 or 10 μg/mL | | Incubation Time: | 24 hours | | Result: | Decreaseed MT-CO1 protein content with concentrations of 1 μg/mL and higher. |
Cell Proliferation Assay[4] | Cell Line: | MCF 12A, 293T cells | | Concentration: | 100 ng/mL, 1 μg/mL | | Incubation Time: | 96 hours | | Result: | Caused reduced proliferation of MCF 12A and 293T cells at 1 μg/mL. |
Cell Viability Assay[5] | Cell Line: | MCF-7, MDA-MB-468 cells | | Concentration: | 0-250 μM | | Incubation Time: | 72 hours | | Result: | Inhibited breast cancer cells in a dose-dependent manner with IC50values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively. |
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体内研究
(In Vivo) | Doxycycline hydrochloride (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner[3].
| Animal Model: | 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3] | | Dosage: | 200 or 800 mg/kg | | Administration: | Oral gavage; 200 or 800 mg/kg; once daily; 3 months | | Result: | Reduced MMP-9 activity in a dose-dependent manner. |
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| Clinical Trial | |
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| 中文名称 | 盐酸多西环素；盐酸强力霉素盐；多西环素盐酸盐；去氧土霉素盐酸盐 |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
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