Crystal violet (Basic Violet 3) is a triarylmethane dye. Crystal Violet (Gentian Violet) has antiviral effects againstH1N1and also has prominent bactericidal activities.

Crystal violet (Basic Violet 3) is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines^[1][2][3].

407.98

Solid

C₂₅H₃₀ClN₃

548-62-9

结晶紫；龙胆紫；甲基紫

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : ≥ 100 mg/mL(245.11 mM)

H₂O : 5 mg/mL(12.26 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

  Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (6.13 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

  Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (6.13 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90%corn oil

  Solubility: ≥ 2.5 mg/mL (6.13 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (6.13 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• 
  Description: Crystal Violet is a triarylmethane dye and it can be used in migration, invasion and wound-healing assays with violet labeled cells.
  Method: For cell staining.
  1. Fix migratory and invasive cells on the bottom surface of the filters in 4% paraformaldehyde.
  2. Stain cells with crystal violet solution (0.1%).
  3. Quantify cells under an inverted microscope (Olympus, Tokyo, Japan).
• 
  Description: Crystal Violet is a triarylmethane dye and it can be used in colony formation assay with violet labeled cells.
  Method: For colony formation assay.
  1. 3×10²cells are seeded in 6-well plate for 14 days, after being fixed with 4% paraformaldehyde.
  2. Stain cells with 0.1% crystal violet.
  3. Quantify and identify cells.
• 
  Description: Crystal Violet is a triarylmethane dye and it can be used in cell colony assay with violet labeled cells.
  Method: For colony formation assay.
  1. Treat cultured cells with 4% paraformaldehyde.
  2. Stain cells with crystal violet (0.1%).
  3. Cell colonies are calculated in every experiment group.
• 
  Description: Crystal Violet is a triarylmethane dye and it can be used in invasion and migration assays with violet labeled cells.
  Method: For cell staining.
  1. Induce the migration or invasion of testing cells and for the invasion assay, Matrigel (1:6 dilution) is also added to the upper chamber.
  2. Incubate cells with crystal violet (24 h; 37?C; 5% CO₂).
  3. Count cells under an optical microscope.
• 
  Description: Crystal Violet is a triarylmethane dye and it can be used in colony formation assay with violet labeled cells.
  Method: For colony formation assay.
  1. Culture testing cells until colonies with more than 50 cells formed, so the culture is terminated.
  2. Fix cells in 4% paraformaldehyde.
  3. Stain cells with crystal violet and photograph after rinsing with double distilled water.