O4I2 是有效的Oct3/4诱导剂。O4I2 可诱导多能干细胞相关基因Lin28、Sox2和Nanog的表达，并抑制Rex1。
| 生物活性 | O4I2 is a potentOct3/4inducer. O4I2 induces the expression of pluripotent-associated genesLin28,Sox2andNanog, and suppressesRex1[1]. |
体外研究
(In Vitro) | O4I2 not only promotes Oct3/4 expression by activating its promoter site, but also stabilize the Oct3/4 protein in HEK293 cells[1].
O4I2 markedly stimulates Oct3/4 even at 5 μM after 72 hrs treatment on the translational
level[1].
O4I2 (20 μM) accumulates Oct3/4 in NCCIT occurred already 2 hrs after incubation, while the detectable Sox2 increase appears much later, implicating compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal carcinoma NCCIT cells[1].
Immunofluorescence[1] | Cell Line: | HF (human fibroblasts) cells. | | Concentration: | 5-20 μM. | | Incubation Time: | 5 days. | | Result: | Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 42 mg/mL(148.54 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 3.5367 mL | 17.6835 mL | 35.3669 mL | | 5 mM | 0.7073 mL | 3.5367 mL | 7.0734 mL | | 10 mM | 0.3537 mL | 1.7683 mL | 3.5367 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: 2.5 mg/mL (8.84 mM); Suspended solution; Need ultrasonic
此方案可获得 2.5 mg/mL (8.84 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
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