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bpV(phen)trihydrate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
bpV(phen)trihydrate图片
CAS NO:171202-16-7
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
bpV(phen) trihydrate,一种胰岛素模拟物,是一种有效的蛋白酪氨酸磷酸酶 (PTP) 和PTEN抑制剂,对PTENPTP-βPTP-1BIC50为 38 nM,343 nM 和 920 nM。bpV(phen) trihydrate 在体外抑制原生动物寄生虫利什曼原虫的增殖。bpV(phen) trihydrate 强烈诱导大量趋化因子和促炎性细胞因子的分泌,并激活Th1型途径 (IL-12,IFNγ)。bpV(phen) trihydrate 还可以诱导细胞凋亡 (apoptosis),并具有抗血管生成和抗肿瘤活性。
生物活性

bpV(phen) trihydrate, a insulin-mimetic agent, is a potentprotein tyrosinephosphatase(PTP)andPTENinhibitor withIC50s of 38 nM, 343 nM and 920 nM forPTEN,PTP-βandPTP-1B, respectively. bpV(phen) trihydrate inhibits proliferation of the protozoanparasiteLeishmaniain vitro. bpV(phen) trihydrate strongly induces the secretion of a large number of chemokines and pro-inflammatory cytokines, and it activates aTh1-typepathway (IL-12, IFNγ). bpV(phen) trihydrate can also induce cellapoptosis, and has anti-angiogenic and anti-tumor activity[1][2][3][4][5].

IC50& Target

IC50: 38 nM (PTEN), 343 nM (PTP-β) and 920 nM (PTP-1B)[3]
ParasiteLeishmania[2]
Apoptosis[1]

体外研究
(In Vitro)

bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment causes a further decrease of cell viability in H/R-injured H9c2 cells[1].
bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment increases the apoptosis of H/R-injured H9c2 cells[1].
bpV(phen) (5 μM; 24.5 hours; H9c2 cells) treatment significantly promotes the accumulation of cytoplasmic Cytochrome C in H/R-injured H9c2 cells[1].
After stimulation of bpV(phen), PTEN-induced putative kinase protein 1 (PINK1)/Parkin-mediated mitophagy is inhibited[1].
bpV(phen) is an insulin-mimetic agent following insulin-receptor tyrosine kinase hyperphosphorylation and activation[4].

Cell Viability Assay[1]

Cell Line:Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:5 μM
Incubation Time:24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:Caused a further decrease of cell viability.

Apoptosis Analysis[1]

Cell Line:Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:5 μM
Incubation Time:24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:Increased the apoptosis of H/R-injured H9c2 cells.

Western Blot Analysis[1]

Cell Line:Hypoxia/reoxygenation (H/R)-injured H9c2 cells
Concentration:5 μM
Incubation Time:24.5 hours (hypoxia for 24 h; reoxygenation for 30 minutes)
Result:Showed an increased release of Cytochrome C.
体内研究
(In Vivo)

bpV(phen) (5 mg/kg; intraperitoneal injection; daily; for 38 days; male BALB/c nude (nu/nu) athymic mice) treatment causes a significant reduction in average tumor volume[1].

Animal Model:Male BALB/c nude (nu/nu) athymic mice (6-7 weeks old) injected with PC-3 cells[2]
Dosage:5 mg/kg
Administration:Intraperitoneal injection; daily; for 38 days
Result:Caused a significant reduction in average tumor volume.
分子量

404.29

性状

Solid

Formula

C12H14KN2O8V

CAS 号

171202-16-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O : 18.18 mg/mL(44.97 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.4735 mL12.3674 mL24.7347 mL
5 mM0.4947 mL2.4735 mL4.9469 mL
10 mM0.2473 mL1.2367 mL2.4735 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 25 mg/mL (61.84 mM); Clear solution; Need ultrasonic and warming

*以上所有助溶剂都可在本网站选购。