Torin 1 is a potent inhibitor ofmTORwith anIC₅₀of 3 nM. Torin 1 inhibits bothmTORC1/2complexes withIC₅₀values between 2 and 10 nM. Torin 1 is an effective inducer ofautophagy.

Torin1 (250 nM) completely inhibits proliferation and causes a G1/S cell cycle arrest, and decreases cell size to a greater degree than 50 nM rapamycin in wild-type MEFs^[1]. Torin1 has more than 800-fold selectivity between mTOR and PI3Kis, and is very selective relative to other PIKK family kinases with the exception of DNA-PK^[2].

Torin1 (20 mg/kg, i.p.) is efficacious in a U87MG xenograft model, and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues^[2].

607.62

Solid

C₃₅H₂₈F₃N₅O₂

1222998-36-8

Room temperature in continental US; may vary elsewhere.

DMSO : 2 mg/mL(3.29 mM;ultrasonic and warming and heat to 60℃)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 5% DMSO    40%PEG300   5%Tween-80   50% saline

  Solubility: ≥ 0.25 mg/mL (0.41 mM); Clear solution
• 2.

  请依序添加每种溶剂： 5% DMSO    95% (20%SBE-β-CDin saline)

  Solubility: ≥ 0.25 mg/mL (0.41 mM); Clear solution
• 3.

  请依序添加每种溶剂： 1% DMSO    99% saline

  Solubility: 0.05 mg/mL (0.08 mM); Suspended solution; Need ultrasonic