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Zinc Protoporphyrin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Zinc Protoporphyrin图片
CAS NO:15442-64-5
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议

产品名称
锌原卟啉
Zn(II)-protoporphyrin IX
ZnPP
Zinc Protoporphyrin-9
产品介绍
Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) 是一种具有口服活性的,竞争性的血红素加氧酶-1 (HO-1) 抑制剂,显着减弱间苯三酚 (PG) 对 H2O2的保护作用。Zinc Protoporphyrin 可用作个体孕妇和儿童缺铁的筛查标志物,也用于评估人群铁状态与血红蛋白浓度的组合。Zinc Protoporphyrin 具有抗癌活性。
生物活性

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX) is an orally active and competitiveheme oxygenase-1 (HO-1)inhibitor and markedly attenuates the protective effects of Phloroglucinol (PG) against H2O2[1]. Zinc Protoporphyrin is used as a screening marker of iron deficiency in individual pregnant women and children, but also to assess population iron status in combination with haemoglobin concentration[2]. Zinc Protoporphyrin has anti-cancer activity[3].

IC50& Target[1][2]

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Zinc Protoporphyrin (Zn(II)-protoporphyrin IX; 5 μM; 72 hours) causes the fraction of late apoptotic and necrotic cells increasing from 10.9% in controls to 30.4% after 72 h[3].
Zinc Protoporphyrin (1.25-40 μM; 48 or 72 hours) exerts cystostatic/cytotoxic effects against tumor cells[3].
Zinc Protoporphyrin (2.5, 5 μM; 48 or 72 hours) results in dose- and time-dependent reduction of cells in G1 phase of the cell cycle[3].
Zinc Protoporphyrin (1.25-40 μM; 48 hours) leads to accumulation of cleaved (active) caspase-3[3].

Apoptosis Analysis[3]

Cell Line:C-26 cells
Concentration:5 μM
Incubation Time:72 hours
Result:The fraction of late apoptotic and necrotic cells increased from 10.9% in controls to 30.4% after 72 h.

Cell Cytotoxicity Assay[3]

Cell Line:C-26 and MDA-MB231 cells
Concentration:1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time:48 or 72 hours
Result:Exerted cystostatic/cytotoxic effects against tumor cells.

Cell Cycle Analysis[3]

Cell Line:C-26 cells
Concentration:2.5, 5 μM
Incubation Time:48 or 72 hours
Result:Resulted in dose- and time-dependent reduction of cells in G1 phase of the cell cycle.

Western Blot Analysis[3]

Cell Line:C-26 cells
Concentration:1.25, 2.5, 5, 10, 20, 40 μM
Incubation Time:48 hours
Result:Leaded to accumulation of cleaved (active) caspase-3.
体内研究
(In Vivo)

Zinc Protoporphyrin (12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.; from day 7 to 19) exerts dose-dependent antitumor effects manifested by the retardation of tumor growth[3].

Animal Model:BALB/c mice inoculated with C-26 cells[3]
Dosage:12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o.
Administration:IP or PO; from day 7 to 19
Result:Exerted dose-dependent antitumor effects manifested by the retardation of tumor growth.
分子量

626.02

性状

Solid

Formula

C34H32N4O4Zn

CAS 号

15442-64-5

中文名称

锌原卟啉

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : 20.83 mg/mL(33.27 mM;ultrasonic and warming and heat to 80℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.5974 mL7.9870 mL15.9739 mL
5 mM0.3195 mL1.5974 mL3.1948 mL
10 mM0.1597 mL0.7987 mL1.5974 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 50%PEG300   50% saline

    Solubility: 1.67 mg/mL (2.67 mM); Suspended solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。