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Geranylgeraniol
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Geranylgeraniol图片
CAS NO:24034-73-9
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
100mg电议
200mg电议
500mg电议

产品名称
香叶基香叶醇;基牻牛儿醇
产品介绍
Geranylgeraniol 是一种口服有效的维生素 K2亚型,是甲戊酸途径 (mevalonate pathway) 的中间体。Geranylgeraniol 靶向NF-kB信号通路,可在动物模型中减轻脂多糖 (LPS) 诱导的小胶质细胞炎症。
生物活性

Geranylgeraniol is an orally acitve vitamin K2sub-type, an intermediate of the mevalonate pathway. Geranylgeraniol targetsNF-kBsignaling pathway and could alleviate LPS-induced microglial inflammation in animal model[1][2][3][4].

IC50& Target

NF-kB[1]

体外研究
(In Vitro)

Geranylgeraniol (0-10 μM; 24 h) dose-dependently suppresses the LPS-induced increase in the mRNA levels of Il-1β, Tnf-α, Il-6, and Cox-2[1].
Geranylgeraniol (10 μM; 24 h) inhibits the phosphorylation of TAK1, IKKα/β, and NF-κB p65 proteins as well as NF-κB nuclear translocation induced by LPS while maintaining IκBα expression[1].
Geranylgeraniol, (50 μM; 24 h) eliminates cell damage caused bySimvastatin(HY-17502) (10 μM) and Mevalonat (10 mM), and reduces the inflammatory marker and the damage of the mitochondria, maintaining its shape and component[2].

Western Blot Analysis[1]

Cell Line:MG6 cell
Concentration:0, 1, 10 μM
Incubation Time:24 hours
Result:Suppressed by TAK1, IKKα/β, and NF-κB p65 proteins level at 10 μM.

RT-PCR[1]

Cell Line:MG6 cell
Concentration:10 μM
Incubation Time:0, 6, 12, 24 hours
Result:Significantly inhibited pro-inflammatory cytokine Il-1β, Tnf-α, Il-6, and Cox-2 mRNA level.
体内研究
(In Vivo)

Geranylgeraniol (725 mg/kg/d; p.o.; 90 d) is not toxicologically significant with a dose below 725 mg/kg/d in rats[3].
Geranylgeraniol (483 mg/kg/d; p.o.; 10 d) suppresses lipopolysaccharide-induced inflammation via inhibition of nuclear factor-κB activation in rats[4].

Animal Model:Han Wistar rats (169-192 g for male; 116-152 g for female)[3]
Dosage:0, 725, 1450, and 2900 mg/kg
Administration:Oral gavage; once daily; 90 days
Result:Showed the lowest observed adverse effect level (LOAEL) for local effects and the no observed adverse effect level (NOAEL) for systemic effects as 725 mg/kg/d.
Reduced body weights by 12.9 and 21.6% in the intermediate- and high-dose group males, respectively, compared to controls.
Animal Model:Wistar rats (male, 8-week-old, 130-150 g)[4]
Dosage:0, 48.3, 483, 4830 mg/kg
Administration:Oral gavage; once daily; 10 days; with or not LPS challenge (i.p.; 0.5 mg/kg)
Result:Suppressed LPS-induced inflammatory cytokines and mRNA expression of LPS-induced inflammatory genes in liver with doses of 483 mg/kg and 4830 mg/kg.
Suppressed protein levels of IRAK1, TRAF6, and TAK1, originating from transcriptional down-regulation with doses of 483 mg/kg and 4830 mg/kg.
分子量

290.48

性状

Liquid

Formula

C20H34O

CAS 号

24034-73-9

中文名称

香叶基香叶醇;基牻牛儿醇

结构分类
  • Others
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Pure form-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL(344.26 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.4426 mL17.2129 mL34.4258 mL
5 mM0.6885 mL3.4426 mL6.8852 mL
10 mM0.3443 mL1.7213 mL3.4426 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.61 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Suspended solution

    此方案可获得 ≥ 2.5 mg/mL (8.61 mM,饱和度未知) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.61 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。