MIND4-17 是一种有效的NRF2激活剂，可共价修饰 Keap1 的 C151 残基。MIND4-17 破坏 Keap1-Nrf2 结合，导致 Nrf2 蛋白稳定和核易位。MIND4-17 具有强大的抗氧化活性。
| 生物活性 | MIND4-17 is a potentNRF2activator that covalently modifies a C151 residue of Keap1. MIND4-17 disruptsKeap1-Nrf2association, leading to Nrf2 protein stabilization and nuclear translocation. MIND4‐17 exerts potent antioxidant activity[1][2]. |
体外研究
(In Vitro) | MIND4-17（0.1-2 μM；24 小时）以浓度依赖性显着增加典型 ARE 基因 Nqo1、Hmox1、Srx1 的表达，并在较小程度上增加 Gclc[1]。
MIND4-17（0.1-2 μM；24 小时）在 WT 和 HD 突变体 ST14A 细胞中显示浓度依赖性诱导 NQO1 和 GCLM 蛋白[1]。
MIN4-17 (0.1-10 μM) 降低小胶质细胞中的 ROS 水平和氮中间产物的产生[1]。
RT-PCR[1] | Cell Line: | Rat corticostriatal neuronal | | Concentration: | 1 μM, 5 μM | | Incubation Time: | 4 hours, 16 hours | | Result: | Significantly and concentration-dependently increased the expression of the canonical ARE genes Nqo1, Hmox1, Srx1, and to a lesser degree Gclc. |
Western Blot Analysis[1] | Cell Line: | Wild-type (WT) and Huntington's disease (HD) mutant ST14A cells | | Concentration: | 0.1 μM, 0.2 μM, 0.5 μM, 0.7 μM, 1 μM, 1.5 μM, 2 μM | | Incubation Time: | 24 hours | | Result: | Showed a concentration-dependent induction of NQO1 and GCLM proteins. |
|
体内研究
(In Vivo) | MIND4-17（2 mg/kg；玻璃体内注射；一次）激活 Nrf2 信号并减轻小鼠光损伤引起的视网膜功能障碍[2]。
| Animal Model: | BALB/c mice bearing white fluorescent light exposure[2] | | Dosage: | 2 mg/kg | | Administration: | Intravitreal injection; once | | Result: | Activated Nrf2 signaling and attenuated retinal dysfunction by light damage in mice. |
|
| 分子量 | |
| Formula | |
| CAS 号 | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
m.cnreagent.com