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Thioflavin T
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Thioflavin T图片
CAS NO:2390-54-7
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
500mg电议
5 g电议
10 g电议
50 g电议

产品名称
硫黄素T
Basic Yellow 1
产品介绍
Thioflavin T 是一种阳离子 Benzothiazole 染料,在组织切片中与淀粉样蛋白结合后荧光强度增强。
生物活性

Thioflavin T is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections.

体外研究
(In Vitro)

Thioflavin T (ThT) is a benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3.The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission[1].

分子量

318.86

性状

Solid

Formula

C17H19ClN2S

CAS 号

2390-54-7

中文名称

硫黄素T

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 16.67 mg/mL(52.28 mM;Need ultrasonic)

H2O : 12.5 mg/mL(39.20 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.1362 mL15.6809 mL31.3617 mL
5 mM0.6272 mL3.1362 mL6.2723 mL
10 mM0.3136 mL1.5681 mL3.1362 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

染色示例
  • Description: Thioflavin T is a fluorescent dye used for staining Aβ fibrils with green fluorescence. Thioflavin T is weakly fluorescent in aqueous solutions but exhibits an enhanced emission at 482 nm upon binding to Aβ plaques. Thioflavin T can be applied to the research of Alzheimer disease.
  • Description: Thioflavin T can be used to detect endoplasmic reticulum stress (ERS) by exhibiting fluorescence when it binds to protein aggregates with green fluorescence.
    Method: For dye binding assay.
    1. Cells are fixed in 4% paraformaldehyde, permeabilized by 0.1% Triton X/PBS, washed for 3 times.
    2. Incubate cells with Thioflavin T (5 μM; 20 min; 37℃).
    3. Wash cells for three times with PBS, mount cells to slides using two drops of Fluoromount and stored at 4℃ in the dark.
    4. Confocal images are captured using an automatic microscope (BX63, Olympus Corp).