Bz-FVR-AMC is a fluorogenic substrate forprocathepsinwith a k_cat/K_mvalue of 1070 mM^-1s^-1. The high concentration of BZ-FVR-AMC inhibits the substrate^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Processing and activation of procathepsin S^[2]:
1. Autocatalytic activation of procathepsin S was studied by incubation (final concentration 1-5 μM) at 37 ℃ in 0.5 mL of the appropriate buffer containing 2.5 mM dithiothreitol.
2. Aliquots of 10 μl were taken from the reaction mixture at the times indicated and mixed with 2.5 ml of substrate solution (10 μMBz-FVR-AMCin 0.1 M phosphate buffer, pH 6.5, containing 1 mMEDTA(HY-Y0682) and 0.1% (w/v) polyethylene glycol 6000).
3. Fluorescence of the released AMC was then monitored continuously for 1 min in a pectrofluorimeter at excitation and emission wavelengths of 370 and 460 nm.

681.78

C₃₇H₄₃N₇O₆

88899-22-3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.