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Cycloleucine
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Cycloleucine图片
CAS NO:52-52-8
包装与价格:
包装价格(元)
25mg电议
50mg电议
100mg电议

产品名称
环亮氨酸
产品介绍
Cycloleucine 是一种 S-腺苷甲硫氨酸介导的甲基化的特异性抑制剂。Cycloleucine 是NMDA受体甘氨酸变构位点的拮抗剂,Ki值为 600 μM。Cycloleucine 在体外也是 ATP:L-蛋氨酸-S-腺苷基转移酶的竞争性抑制剂。Cycloleucine 具有抗焦虑和抑制细胞作用。
生物活性

Cycloleucine is a specific inhibitor of S-adenosyl-methionine mediated methylation. Cycloleucine is antagonist ofNMDAreceptor associated glycine receptor, with aKiof 600 μM. Cycloleucine is also a competitive inhibitor of ATP: L-methionine-S-adenosyl transferase in vitro. Cycloleucine has anxiolytic and cytostatic effects[1][2][3][4].

IC50& Target

Ki: 600 μM (NMDA)[1][2]

体外研究
(In Vitro)

Cycloleucine (4-40 mM; 3 h) blocks internal methylation of viral RNA in B77 transformed chick embryo fibroblasts[5].
Cycloleucine (40 mM; 24 h) blocks the formation of both m6A and the penultimate Gmin B77 38S RNA subunits by greater than 90%[5].
Cytostatic (10 μg/mL) inhibits the viability human KB and mouse L1210s leukemia cell lines[5].

体内研究
(In Vivo)

Cycloleucine (0.5-4 μg; intracerebroventrical injection) increases time spent in open arms, open arm entries, and extreme arrivals in rats[3]. Cycloleucine reduces thymus and spleen weights inSemliki Forestvirus (SFV) strain A7(74) infected and control mice[6].

Animal Model:Male rats bilaterally cannulated into the nucleus accumbens septi (NAS)[3]
Dosage:1 μL of 0.5, 1.0, 2.0, 4 μg/μL
Administration:Intracerebroventrical injection
Result:Increased time spent in the open arms and extreme arrivals at all doses.
Increased open arm entries at the dose of 4 μg.
分子量

129.16

性状

Solid

Formula

C6H11NO2

CAS 号

52-52-8

中文名称

环亮氨酸;1氨基环戊烷甲酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

H2O : 20 mg/mL(154.85 mM;Need ultrasonic)

DMSO :< 1 mg/mL (ultrasonic)(insoluble or slightly soluble)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM7.7423 mL38.7117 mL77.4233 mL
5 mM1.5485 mL7.7423 mL15.4847 mL
10 mM0.7742 mL3.8712 mL7.7423 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (774.23 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。