Avicularin

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Avicularin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

572-30-5

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
25mg	电议
50mg	电议
100mg	电议

产品名称

扁蓄苷；蓄苷

产品介绍

Avicularin 是一种具有口服活性的黄酮类化合物。Avicularin 抑制NF-κB (p65),COX-2和PPAR-γ的活性。Avicularin 具有抗炎、抗感染、抗过敏、抗氧化、保肝、抗肿瘤等作用。

生物活性

Avicularin is an orally active flavonoid. Avicularin inhibitsNF-κB(p65),COX-2andPPAR-γactivities. Avicularin has anti-inflammatory, anti-infectious anti-allergic, anti-oxidant, hepatoprotective, and anti-tumor activities^[1]^[3].

IC₅₀& Target^[2]^[3]

GLUT4

PPARγ

COX-2

体外研究
(In Vitro)

Avicularin (10-300 μM, 1 h) suppresses NO and PGE₂production in LPS-stimulated RAW 264.7 cells^[1].
Avicularin (10-300 μM, 1 h) exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 cells^[1].
Avicularin (25-100 μg/mL, 48 h) decreases cell proliferation, cell migration and invasion in Huh7 cells^[2].
Avicularin (25-100 μg/mL, 48 h) induces apoptosis via the downregulation of NF-κB (p65) and COX-2 and the upregulation of PPAR-γ^[2].
Avicularin (50 μM, 6 days) decreases the intracellular lipids, along with decreased PPARγ, C/EBPα, and aP2 mRNA levels in 3T3-L1 cells^[3].
Avicularin (50 μM, 6 days) suppresses GLUT4-Mediated glucose uptake in 3T3-L1 cells^[3].
Avicularin (2.5-10 μM, 2 h) inhibits ECM degradation and inflammation via TRAF6/MAPK activation in rat and human chondrocytes^[4].

Cell Proliferation Assay^[2]

Cell Line:	Huh7 cells
Concentration:	25, 50, 100 μg/mL
Incubation Time:	12, 24, 36 and 48 h
Result:	Inhibited cell proliferation in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	RAW 264.7 cells
Concentration:	10, 30, 100, 300 μM
Incubation Time:	1 h
Result:	Inhibited LPS-induced protein expression of iNOS and COX-2, release of pro-inflammatory cytokine IL-1β, degradation of cytosolic IκB, and phosphorylation of ERK.

RT-PCR^[3]

Cell Line:	3T3-L1 cells
Concentration:	50 μM
Incubation Time:	6 days
Result:	Decreased PPARγ, C/EBPα, and aP2 mRNA levels approximately 28.7, 69.5, and 18.3%, respectively.

体内研究
(In Vivo)

Avicularin (injected in articular cavity of the knee, 0.5-2 mg/kg, twice a week for 4 weeks) attenuates the development of OA (osteoarthritis) in ACLT-induced rats^[4].
Avicularin (oral administration, 50 and 100 mg/kg, for 21 days) attenuates memory Impairment in rats with amyloid Beta-induced Alzheimer's disease^[5].

Animal Model:	ACLT (anterior cruciate ligament transection)-induced rats^[4]
Dosage:	0.5, 1, 2 mg/kg
Administration:	Injected into the articular cavity of the right knee, twice a week for 4 weeks.
Result:	Alleviated the tibial subchondral osteolysis, reduces bone loss, and increases the bone mass of tibial subchondral bone. Attenuated ECM degradation, the loss of Aggrecan and Collagen II in ACLT-induced rats. Decreased the MMP3 and MMP13 protein level.

Animal Model:	Rats with amyloid Beta-induced Alzheimer's disease^[5]
Dosage:	25, 50, and 100 mg/kg
Administration:	Oral administration, for 21 days
Result:	Enhanced cognition activity, and reversed the effects of amyloid beta-induced inflammatory response and excessive oxidative stress.

分子量

434.35

性状

Solid

Formula

C₂₀H₁₈O₁₁

CAS 号

572-30-5

中文名称

扁蓄苷；蓄苷

结构分类

Flavonoids
Flavonols

Phenols
Polyphenols

来源

Plants
Lauraceae
Lindera aggregata(Sims) Kosterm.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL(230.23 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.3023 mL	11.5115 mL	23.0229 mL
5 mM	0.4605 mL	2.3023 mL	4.6046 mL
10 mM	0.2302 mL	1.1511 mL	2.3023 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (4.79 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
2.
请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline)
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution
此方案可获得 ≥ 2.08 mg/mL (4.79 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在本网站选购。