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γ-Aminobutyric acid
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
γ-Aminobutyric acid图片
CAS NO:56-12-2
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
100mg电议
200mg电议
500mg电议

产品名称
γ-氨基丁酸
4-Aminobutyric acid
产品介绍
γ-Aminobutyric acid (4-Aminobutyric acid) 是成年哺乳动物大脑中主要的抑制性神经递质,能够与离子移变GABAA受体和促代谢GABAB受体结合发挥作用。γ-Aminobutyric acid 能够阻断中枢神经系统的特定信号,并带来镇静效果。
生物活性

γ-Aminobutyric acid (4-Aminobutyric acid) is a major inhibitory neurotransmitter in the adult mammalian brain, binding to the ionotropic GABA receptors (GABAAreceptors) and metabotropic receptors (GABABreceptors. γ-Aminobutyric acid shows calming effect by blocking specific signals of central nervous system[1][2].

IC50& Target

Human Endogenous Metabolite

 

Microbial Metabolite

 

体外研究
(In Vitro)

γ-Aminobutyric acid (30 μM) depolarizes cortical progenitor cells (E16 cells), results an inward current in ventricular zone (VZ) cells, and induces DNA synthesis inhibition, with half-maximal response concentration of 5 μM[3].
Cortical plate (cp) neurons expresses glutamic acid decarboxylase (GAD), γ-Aminobutyric acid (1-5 μM; 18 h) stimulates the motility and arrests the migration of cp cells, while the chemotropic signal is involved G-protein activation[4].
γ-Aminobutyric acid activates GABAAreceptors, causing cell cycle arrest in S phase and limiting growth[5].

Cell Migration Assay[4]

Cell Line:Cortical plate (cp) neurons
Concentration:1-5 μM
Incubation Time:18 hours
Result:Promoted motility via G-protein activation and arrested attractantinduced migration via GABAA receptor-mediated depolarization.
体内研究
(In Vivo)

γ-Aminobutyric acid (33.95, 102.25, 306.75 mg/kg; p.o.; single dose) can enhance the sleep of mice[6].
γ-Aminobutyric acid (1, 2, 4 mg/kg/d; p.o.; 30 d) alleviates anxiety and restored food utilization rate in rats, with impairment induced by Di(2-ethylhexyl) phthalate (DEHP) exposure[7].

Animal Model:Pathogens free (SPF) Bagg’s albino (Balb/c) mice (18–20 g, 8 weeks old)[6]
Dosage:33.95, 102.25, 306.75 mg/kg
Administration:Oral gavage; single dose; 20 mL/kg administration; measured in an hour
Result:Prolonged the sleep duration, increased sleep rate, and shorten the sleep latency more effectively.
Animal Model:Sprague-Dawley rat indued byDEHP(HY-B1945) (500 mg/kg)[7]
Dosage:1, 2, 4 mg/kg
Administration:Oral gavage; combined administration; for 30 consecutive days
Result:Reduced the levels of nitric oxide and nitric oxide synthase in rats treated with DEHP.
分子量

103.12

性状

Solid

Formula

C4H9NO2

CAS 号

56-12-2

中文名称

γ-氨基丁酸

结构分类
  • Ketones, Aldehydes, Acids
来源

mammalian central nervous system

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

H2O : 50 mg/mL(484.87 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM9.6974 mL48.4872 mL96.9744 mL
5 mM1.9395 mL9.6974 mL19.3949 mL
10 mM0.9697 mL4.8487 mL9.6974 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (969.74 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。