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Norartocarpetin
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Norartocarpetin图片
CAS NO:520-30-9
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
25mg电议

产品介绍
Norartocarpetin 是一种酪氨酸酶抑制剂。Norartocarpetin 具有较强的酪氨酸酶抑制活性,其抑制活性IC50值为 0.47 μM。Norartocarpetin 作为一种抗褐变剂可用于食品体系的研究。Norartocarpetin 对肺癌细胞 (NCI-H460) 也具有显著的抗癌活性,其IC50值为 22 μM。Norartocarpetin 的抗增殖作用是通过靶向 Ras/Raf/MAPK 信号通路、线粒体介导的细胞凋亡、S 期细胞周期阻滞和抑制细胞迁移和侵袭实现的。
生物活性

Norartocarpetin is atyrosinaseinhibitor. Norartocarpetin has strongtyrosinaseinhibitory activity with anIC50value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with anIC50value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediatedapoptosis, S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells[1][2].

IC50& Target

IC50: 0.47 μM (tyrosinase);[1]IC50: 22 μM (NCI-H460 cells); 85 μM (MRC-9 cells)[2]

体外研究
(In Vitro)

Norartocarpetin has strong mushroom tyrosinase inhibitory activity with anIC50value of 0.47 μM[1].
Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC50values of 22 μM and 85 μM, respectively[2].
Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460)[2].
Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest[2].
Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion[2].
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration[2].

Cell Viability Assay[2]

Cell Line:MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells)
Concentration:0, 3.12, 6.25, 12.5, 25, 50, and 100 μM
(first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min)
Incubation Time:48 h
Result:Repressed the viability of NCI-H460 cells in a dose-dependent manner.

Western Blot Analysis[2]

Cell Line:MRC-9 and NCI-H460 cells
Concentration:0, 11, 22, and 44 μM
Incubation Time:24 h
Result:Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38.

Apoptosis Analysis[2]

Cell Line:MRC-9 and NCI-H460 cells
Concentration:0. 11, 22 and 44 μM
Incubation Time:24 h
Result:Led to dose-dependent apoptosis and induced DNA damage.

Cell Cycle Analysis[2]

Cell Line:MRC-9 and NCI-H460 cells
Concentration:0, 11, 22, and 44 μM
Incubation Time:
Result:Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively.

Cell Invasion AssayMRC-9 and NCI-H460 cells

Cell Line:MRC-9 and NCI-H460 cells
Concentration:22 μM
Incubation Time:24 h
Result:Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells.

Cell Migration Assay[2]

Cell Line:MRC-9 and NCI-H460 cells
Concentration:0, 11, 22, and 44 μM
Incubation Time:24 h
Result:Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.
分子量

286.24

性状

Solid

Formula

C15H10O6

CAS 号

520-30-9

结构分类
  • Flavonoids
  • Flavones
来源
  • Plants
  • Moraceae
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 100 mg/mL(349.36 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.4936 mL17.4679 mL34.9357 mL
5 mM0.6987 mL3.4936 mL6.9871 mL
10 mM0.3494 mL1.7468 mL3.4936 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。