Cirsilineol

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Cirsilineol

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

41365-32-6

包装与价格：

包装	价格(元)
1mg	电议
5mg	电议
10mg	电议
50mg	电议

产品名称

甲基条叶蓟素

产品介绍

Cirsilineol 是一种天然黄酮化合物，可选择性抑制肠道 CD4⁺T 细胞中的 IFN-γ/STAT1/T-bet 信号。Cirsilineol 具有免疫抑制和抗肿瘤特性。Cirsilineol 显着改善三硝基苯磺酸 (TNBS) 诱导的小鼠 T 细胞介导的实验性结肠炎。

生物活性

Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinalCD4⁺T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice^[1].

体外研究
(In Vitro)

Cirsilineol (0.1-10 μM; 96 hours) inhibits single mixed lymphocyte reaction and Concanava A-induced T-cell proliferation in a dose-dependent manner. Cirsilineol (10 μM) does not affect T lymphocyte's viability. The inhibition of cirsilineol (10 μM) on T-cell proliferation is not due to a cytotoxic activity^[1].
Cirsilineol (1-10 μM; pretreatment for 3 hours) completely inhibits IFN-γ-induced Tyr701 phosphorylation of STAT1 and JAK2 activation^[1].

Cell Proliferation Assay^[1]

Cell Line:	Splenocytes
Concentration:	0.1, 1, 10 μM
Incubation Time:	96 hours
Result:	Inhibited single mixed lymphocyte reaction and Concanavalin A (Con A; 5 μg/mL; for 72 h)-induced T-cell proliferation in a dose-dependent manner. Suppressed OVA_323-339-specific CD4⁺T-cell proliferation.

Western Blot Analysis^[1]

Cell Line:	Splenic CD4⁺T cells
Concentration:	1, 5, 10 μM
Incubation Time:	Pretreatment for 3 hours
Result:	Completely inhibited IFN-γ-induced (25 ng; 30 mins) Tyr701 phosphorylation of STAT1 and JAK2 activation. Suppressed IFN-γ-induced (25 ng; 12 hours) Th1-specific transcription factor T-bet.

体内研究
(In Vivo)

cirsilineol (3, 10, and 30 mg/kg) significantly ameliorates TNBS-induced Th1-mediated colitis through inhibiting IFN-γ/STAT1/T-bet signaling in CD4⁺T cells.

Animal Model:	8-10-week-old female C57BL/6, BALB/c and DO11.10 transgenic mice with TNBS (10 mg; 100 μL)^[1]
Dosage:	3, 10, 30 mg/kg
Administration:	IP; daily; for 11 days
Result:	Showed a significant improved effect on the body weights and survival rate of mice. Markedly reduced inflammatory infiltration, restoration of the destructive mucosal architecture and remission of edema.

分子量

344.32

性状

Solid

Formula

C₁₈H₁₆O₇

CAS 号

41365-32-6

中文名称

甲基条叶蓟素

结构分类

Flavonoids
Flavones

Phenols
Monophenols

来源

Plants
Compositae
Artemisia vestitaWall. ex Bess.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)