Sodium taurodeoxycholate hydrate 是一种胆汁酸,是由肝脏中的胆固醇合成的两亲性表面活性剂分子。 除TGR5通路外,Sodium taurodeoxycholate hydrate 还激活S1PR2通路。
生物活性 | Sodium taurodeoxycholate hydrate, a bile acid, is an amphiphilic surfactant molecule synthesized fromcholesterolin the liver. Sodium taurodeoxycholate hydrate activates theS1PR2pathway in addition to theTGR5pathway[1]. |
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体外研究 (In Vitro) | The median plasma concentration of Taurodeoxycholate is 33.9 nM in healthy individuals[1]. Taurodeoxycholate inhibits the binding of N-3H-methylscopolamine to the M3 muscarinic receptor of acetylcholine with an IC50of 170 μM[1]. Taurodeoxycholate (0.05-1.00 mM; 1-6 days) stimulates intestinal epithelial cell proliferation[2]. Taurodeoxycholate (0.05-1.00 mM; 24 h) induces a significant increase in S-phase concentration and a significant decrease in G1-phase concentration of the cell cycle, increases c-myc protein and mRNA expression in IEC-6 cells[2].
Cell Proliferation Assay[2] Cell Line: | IEC-6 and caco-2 cells | Concentration: | 0, 0.05, 0.50, and 1.00 mM | Incubation Time: | 1, 2, 4 and 6 days | Result: | Significantly stimulated intestinal epithelial cell proliferation in a dose-dependent manner. |
Cell Cycle Analysis[2] Cell Line: | IEC-6 cells | Concentration: | 0, 0.05, 0.50, and 1.00 mM | Incubation Time: | 24 h | Result: | Significantly increased cells in S phase and decreased cells in G1-phase. |
Western Blot Analysis[2] Cell Line: | IEC-6 cells | Concentration: | 0.5 mM | Incubation Time: | 1 and 6 days | Result: | Significantly increased c-myc protein expression. |
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体内研究 (In Vivo) | Taurodeoxycholate (0.5 mg/kg; i.v.; once) confers protection to C57BL/6N mice with sepsis, but does not protect TGR5 KO mice under sepsis[1].
Animal Model: | C57BL/6N mice,Lipopolysaccharides(HY-D1056) injection model of sepsis[1] | Dosage: | 0.5 mg/kg | Administration: | Intravenous injection, 30 min or 24 h after LPS injection | Result: | Improved the survival rate of mice with sepsis. Decreased liver and kidney damage in septic mice. Ameliorated systemic inflammation and normalized blood pressure in septic mice. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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