7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptorEBI2 (GPR183). 7α, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC₅₀=140 pM;K_d=450 pM). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells^[1][2].

In vitro, 7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells^[1].

EBI2-deficient B cells or normal B cells desensitized by 7α,25-Dihydroxycholesterol (1 μM; 1.5 hours) pre-treatment shows reduced homing to follicular areas of the spleen^[1].

418.65

Solid

C₂₇H₄₆O₃

64907-22-8

• Steroids

Room temperature in continental US; may vary elsewhere.

DMSO : 4.55 mg/mL(10.87 mM;Need ultrasonic)

Ethanol : 1 mg/mL(2.39 mM;Need ultrasonic)

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