您好，欢迎来到试剂仪器网！ [登录] [免费注册]
试剂仪器网
发布求购
位置：首页 > 产品库 > Sincalide ammonium
立即咨询
咨询类型：
     
*姓名：
*电话：
*单位：
Email：
*留言内容：
请详细说明您的需求。
*验证码：
 
Sincalide ammonium
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Sincalide ammonium图片
CAS NO:70706-98-8
包装与价格：
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
Cholecystokinin octapeptide ammonium
CCK-8 ammonium
SQ19844 ammonium
产品介绍
Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium) 是一种速效胆囊收缩素 (CCK) 的氨基酸多肽激素类似物，在胆囊造影术中静脉使用。Sincalide ammonium 是 CCK 的一个主要生物活性片段，它保留了 CCK 的大部分生物活性。可通过注射促进胆囊收缩并帮助诊断胆囊和胰腺疾病。其增加胆汁的分泌，使胆囊收缩并使 Oddi 的括约肌松弛，从而使胆汁排入十二指肠。
生物活性

Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium) is a rapid-actingamino acid polypeptide hormone analogueof cholecystokinin (CCK) for intravenous use in postevacuation cholecystography. Sincalide ammonium is a major bioactive segment of CCK that retains most of the biological activities of CCK. CCK‐8 can promote gallbladder contraction by injection and helps diagnose gallbladder and pancreas disorders. Sincalide ammonium can increase bile secretion, cause the gallbladder to contract and relax the sphincter of Oddi, resulting in bile drainage into the duodenum. Sincalide ammonium is a major bioactive segment of CCK that retains most of the biological activities of CCK[1][2][3].

体外研究
(In Vitro)

Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium), as a novel cardiovascular hormone, has a significant inhibitory effect on myocardial fibrosis in noninfarcted areas. Sincalide ammonium also plays a positive role in fighting inflammation, apoptosis and collagen deposition. CCK‐8 (ammonium) protects H9c2 cardiomyoblasts from Ang II‐induced apoptosis partly via activation of the CCK1 receptor and the phosphatidyqinositol‐3 kinase/protein kinase B (PI3K/Akt) signaling pathway[3].

Cell Viability Assaysup>[3]

Cell Line:H9c2 cells
Concentration:0.001, 0.01, 0.1, 1, 10, or 100 μmol/L
Incubation Time:24 h
Result:Attenuated Ang II‐induced toxicity in H9c2 cells

Apoptosis Analysis[3]

Cell Line:H9c2 cells
Concentration:0.001, 0.01, 0.1, 1, 10, or 100 μmol/L
Incubation Time:24 h
Result:Decreased apoptotic cells, and prevented Ang II‐induced cytotoxicity that involves modulation of the PI3K/Akt pathway.

Western Blot Analysis[3]

Cell Line:H9c2 cells
Concentration:0.001, 0.01, 0.1, 1, 10, or 100 μmol/L
Incubation Time:24 h
Result:Expressed the protein and mRNAs of CCK and both its receptors in H9c2 cells.

RT-PCR[3]

Cell Line:H9c2 cells
Concentration:0.001, 0.01, 0.1, 1, 10, or 100 μmol/L
Incubation Time:24 h
Result:Increased the protein and mRNA expression levels of CCK and decreased CCK 1 receptor expression levels at both the protein and mRNA levels with Ang II stimulation markedly.
体内研究
(In Vivo)

Sincalide ammonium (Cholecystokinin octapeptide ammonium, CCK-8 ammonium) alleviates fibrosis in the noninfarcted regions and delay the left ventricular remodeling and the progress of heart failure in a MI rat model[4].

Animal Model:MI rat model[4]
Dosage:50 μg/kg
Administration:i.p.; 50 μg/kg/d; for 4 weeks
Result:Had significant inhibitory effect on myocardial fibrosis in noninfarcted areas.
Clinical Trial
分子量

1160.30

性状

Solid

Formula

C49H65N11O16S3

CAS 号

70706-98-8

Sequence Shortening

D-{SO3H-Tyr}-MGWMDF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

H2O : 12.5 mg/mL(10.77 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.8618 mL4.3092 mL8.6185 mL
5 mM0.1724 mL0.8618 mL1.7237 mL
10 mM0.0862 mL0.4309 mL0.8618 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。