[Tyr8] Bradykinin 是一种B2激肽受体 (B2kinin receptor) 激动剂。[Tyr8] Bradykinin 也刺激ERK1/2磷酸化。[Tyr8] Bradykinin 可作为内标。
生物活性 | [Tyr8] Bradykinin is aB2kinin receptoragonist. [Tyr8] Bradykinin also stimulatesERK1/2 phosphorylation. [Tyr8] Bradykinin can be used as an internal standard[1]. |
IC50& Target | Bradykinin B2 Receptor (B2R) | ERK1 | ERK2 |
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体外研究 (In Vitro) | [Tyr8] Bradykinin (10 nM, 2 min) produces a rapid increase in phosphorylated ERK1/2[1].
Western Blot Analysis[1] Cell Line: | Serum-deprived trabecular meshwork cells | Concentration: | 10 nM | Incubation Time: | 2 min | Result: | Produced a rapid increase in phosphorylated ERK1/2 that was equivalent in magnitude to that observed forBradykinin(HY-P0206). |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
Solvent & Solubility | In Vitro: H2O Peptide Solubility and Storage Guidelines: 1. Calculate the length of the peptide. 2. Calculate the overall charge of the entire peptide according to the following table: | Contents | Assign value | Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 | Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 | Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution: Overall charge of peptide | Details | Negative (<0) | 1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. | Positive (>0) | 1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. | Zero (=0) | 1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
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