Elabela(19-32) is an active fragment of ELABELA (ELA) that binds toapelin receptor (APJ). Elabela(19-32) activates theG_αi1andβ-arrestin-2signaling pathways withEC₅₀s of 8.6 nM and 166 nM. Elabela(19-32) induces receptor internalization and reduces arterial pressure, exerts positive inotropic effects on the heart^[1].

IC50: 8.6 nM (G_αi1) and 166 nM (β-arrestin-2)^[1]

Elabela(19-32) (analogue 3) has a K_iof 0.93 nM for binding of radioligand apelin-13[Glp⁶⁵, Nle⁷⁵, Tyr⁷⁷][¹²⁵I]^[1].
Elabela(19-32) has an EC₅₀of 36 nM in HEK293 cells transiently expressing the HA-hAPJ receptor. Elabela(19-32) is slightly less potent than apelin-13 and ELA to elicit receptor internalization^[1].
Elabela(19-32) (0.001 to 0.3 nM) has an EC₅₀of 1.5 pM in inducing changes in left ventricular developed pressure (LVDP) on the Langendorff perfused isolated rat heart^[1].

Elabela(19-32) (analogue 3) is rapidly metabolized in rat plasma (t_1/2<2 min)^[1].

1707.03

Solid

C₇₅H₁₁₉N₂₅O₁₇S₂

1886973-05-2

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

H₂O : 100 mg/mL(58.58 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 50 mg/mL (29.29 mM); Clear solution; Need ultrasonic