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Propranolol hydrochloride
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Propranolol hydrochloride图片
CAS NO:318-98-9
包装与价格：
包装价格(元)
10 mM * 1 mL in DMSO电议
100mg电议
500mg电议
1 g电议
5 g电议
10 g电议

产品名称
盐酸普萘洛尔
产品介绍
Propranolol hydrochloride 是一种非选择性的β-adrenergic receptor (βAR)拮抗剂，对 β1AR 和 β2AR 具有高亲和力，Ki值分别为 1.8 nM 和 0.8 nM。Propranolol hydrochloride 抑制 [3H]-DHA 与大鼠脑膜制剂的结合，IC50为 12 nM。Propranolol hydrochloride 可以用于高血压，嗜铬细胞瘤，心肌梗塞，心律不齐，心绞痛和肥大心肌病的相关研究。
生物活性

Propranolol hydrochloride is a nonselectiveβ-adrenergic receptor (βAR)antagonist, has high affinity for the β1AR and β2AR withKivalues of 1.8 nM and 0.8 nM, respectively[1]. Propranolol hydrochloride inhibits [3H]-DHA binding to rat brain membrane preparation with anIC50of 12 nM[2]. Propranolol hydrochloride is used for study of hypertension, pheochromocytoma, myocardial infarction, cardiac arrhythmias, angina pectoris, and hypertrophic cardiomyopathy[3].

IC50& Target

β adrenergic receptor

 

体外研究
(In Vitro)

Propranolol hydrochloride (10-7M-10-3M; 24 and 48 hours) increases the total ERK1/2 levels in a dose-dependent manner, and ERK1/2 activation is observed specifically at 10-5M in HemSCs[4].
Propranolol hydrochloride (10-9M-10-3M; 24 and 48 hours) significant decreases cell proliferation at 10-4M propranolol after 24 hours and 10-9M propranolol after 48 hours in HemSCs[4].
Propranolol hydrochloride (50 μM-200 μM; 24 hours) increases Annexin V positivity and caspase-3 activation, rapidly induces HemSC apoptosis[4].

Western Blot Analysis[4]

Cell Line:HemSC cells
Concentration:10-7M-10-3M
Incubation Time:24 and 48 hours
Result:Increased the total ERK1/2 levels in a dose-dependent manner.

Cell Viability Assay[4]

Cell Line:HemSC cells
Concentration:10-9M-10-3M
Incubation Time:24 and 48 hours
Result:Suppressed HemSC Proliferation.

Apoptosis Analysis[4]

Cell Line:HemSC cells
Concentration:50 μM, 100 μM, or 200 μM
Incubation Time:24 hours
Result:Induced HemSC cell death occurred via an apoptotic pathway.
体内研究
(In Vivo)

Propranolol hydrochloride (orally administration; 40 mg/kg; daily) significantly reduces the vessel diameter relative to the vehicle-treated implants, and increases the number of cells that expressed phosphorylated ERK1/2 within the IH Matrigel implant[4].

Animal Model:A xenograft mouse model of IH (infantile hemangiomas) with HemSC cells[4]
Dosage:40 mg/kg
Administration:Orally administration; 40 mg/kg; daily
Result:Improved vessel development in the IH mouse model that correlated with MAPK pathway activation.
Clinical Trial
分子量

295.80

性状

Solid

Formula

C16H22ClNO2

CAS 号

318-98-9

中文名称

盐酸普萘洛尔；盐酸普奈洛尔

结构分类
  • Alkaloids
  • Other Alkaloids
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO : ≥ 150 mg/mL(507.10 mM)

H2O : 33.33 mg/mL(112.68 mM;Need ultrasonic)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.3807 mL16.9033 mL33.8066 mL
5 mM0.6761 mL3.3807 mL6.7613 mL
10 mM0.3381 mL1.6903 mL3.3807 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： PBS

    Solubility: 25 mg/mL (84.52 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。