Cholera toxin (Choleragen), an AB(5)-subunit toxin, enters host cells by binding the ganglioside GM1 at the plasma membrane (PM) and travels retrograde through the trans-Golgi Network into the endoplasmic reticulum (ER)^[1]. Choleragen activatesadenylate cyclaseby catalyzing ADP-ribosylation of Gs alpha, the stimulatory guanine nucleotide-binding protein^[2].

In the ER, a portion of Cholera toxin, the enzymatic A1-chain, is unfolded by protein disulfide isomerase and retro-translocated to the cytosol by hijacking components of the ER associated degradation pathway for misfolded proteins^[1].
Cholera toxin acts as an ADP-ribosyltransferase to disrupt intracellular signaling in the target cell. Cholera toxin moves by vesicle carriers from the cell surface to the endoplasmic reticulum (ER) of an intoxicated cell^[3].

Lyophilized Powder

9012-63-9

霍乱毒素

Room temperature in continental US; may vary elsewhere.

Store at 4°C, do not freeze

H₂O : ≥ 10 mg/mL

*"≥" means soluble, but saturation unknown.