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Gap 26
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CAS NO:197250-15-0
包装与价格:
包装价格(元)
1mg电议
5mg电议
10mg电议
50mg电议
100mg电议

产品介绍
Gap 26 是一种连接蛋白 (connexin) 模拟肽,由连接蛋白 connexin 43 (gap junction 阻滞剂)第一个胞外环的第 63-75 残基组成,包含SHVR氨基酸基序。
生物活性

Gap 26 is a connexin mimetic peptide, composed of residue numbers 63-75 of the first extracellular loop of connexin 43 (gap junction blocker), containing the SHVR amino acid motif[1].

IC50& Target

Gap Junction Protein[1]

体外研究
(In Vitro)

Gap 26 (0.25 mg/mL, 30 min) reduces the wave size in the three cell lines (RBE4, SV-ARBEC and ECV304). Gap 26 (0.25 mg/mL, 30 min) completely abolishes the InsP3-triggered ATP response and reduced the ATP release to below the control level, indicating that the basal ATP release is also affected[1].
Gap 26 does indeed significantly inhibit our InsP3-triggered intercellular calcium waves, but it did not have any effect on dye coupling through junctional channels as evidenced by the FRAP experiments, despite the fact that connexin 43 was present in the cell lines used[1].
Gap 26 (100-300 μM) dose-dependently reduces the rhythmic responses of rabbit superior mesenteric arteries, with IC50of 28.4 ± 3.4 μM[2].

分子量

1550.78

性状

Solid

Formula

C70H106N19O19S

CAS 号

197250-15-0

Sequence

Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg

Sequence Shortening

VCYDKSFPISHVR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder-80°C2 years
-20°C1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

H2O : 50 mg/mL(32.24 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM0.6448 mL3.2242 mL6.4484 mL
5 mM0.1290 mL0.6448 mL1.2897 mL
10 mM0.0645 mL0.3224 mL0.6448 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (64.48 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。