Pyridostatin (RR82) TFA 是一种高选择性的G-quadruplex DNA稳定剂 (Kd=490 nM)。Pyridostatin TFA 通过诱导复制和转录依赖的 DNA 损伤促进人类癌细胞生长停滞。Pyridostatin TFA 靶向原癌基因 Src。Pyridostatin TFA 降低人乳腺癌细胞 SRC 蛋白水平和 SRC 依赖的细胞运动。
生物活性 | Pyridostatin (RR82) TFA is aG-quadruplexDNAstabilizing agent (Kd=490 nM). Pyridostatin TFA promotes growth arrest in humancancercells by inducing replication- and transcription-dependent DNA damage. Pyridostatin TFA targets the proto-oncogeneSrc. Pyridostatin TFA reducedSRCprotein levels and SRC-dependent cellular motility in human breastcancercells[1][2][3]. |
体外研究 (In Vitro) | Pyridostatin (RR82) hydrochloride (10 μM; 48 hours) induces cell cycle arrest[1]. Pyridostatin TFA is a very selective G-quadruplex DNA-binding small molecule designed to form a complex with and stabilize G-quadruplex structure. Pyridostatin TFA causes neurite retraction, synaptic loss, and dose-dependent neuronal death. In cultured primary neurons, Pyridostatin TFA induces the formation of DNA DSBs. Remarkably, Pyridostatin TFA (1-5 μM, overnight) downregulates the BRCA1 protein, a protein that guards and repairs the neuronal genome, at the transcriptional level[3].
Cell Viability Assay[1] Cell Line: | Over 60 different cancer cell lines | Concentration: | 10 μM | Incubation Time: | 48 hours | Result: | Predominantly accumulated in the G2 phase of the cell cycle over 60 different cancer cell lines. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |