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CP681301
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
CP681301图片
CAS NO:865317-32-4
包装与价格:
包装价格(元)
5mg电议
10mg电议
25mg电议
50mg电议
100mg电议
200mg电议
500mg电议

产品介绍
CP681301 是一种有效的CDK5抑制剂。CP681301 显示出抗增殖活性。CP681301 降低 GSC 中 CD133、OLIG2、SOX2、KI67、pCDK5 蛋白水平的表达。CP681301 减少小鼠胶质瘤异种移植物的自我更新。CP681301 在果蝇中显示出抗肿瘤活性。
生物活性

CP681301 is a potentCDK5inhibitor. CP681301 shows antiproliferative activity. CP681301 decreases the expression ofCD133, OLIG2, SOX2, KI67, pCDK5 protein level in GSCs (Glioma stem cells). CP681301 reduces self-renewal in mouse glioma xenografts. CP681301 shows anti-tumor activity inDrosophila[1].

体外研究
(In Vitro)

CP681301 (1 μM; 96 h) shows variably cytotoxic to the tested GSC cultures but is not toxic to NHNPs[1].
CP681301 (0, 10, 50 μM; 48 h) decreases the expression of CD133, OLIG2, SOX2, KI67, pCDK5 protein level in GSCs[1].
CP681301 (0, 0.5, 1 μM; ) suppresses the expression of CREB Ser133 phosphorylation[1].

Cell Cytotoxicity Assay[1]

Cell Line:NHNP, N12.159, GBM121, GBM39, N08-74 cells
Concentration:1 μM
Incubation Time:96 h
Result:Showed variably cytotoxic to the tested GSC cultures but was not toxic to NHNPs (normal human neuro-progenitors).

Western Blot Analysis[1]

Cell Line:GBM 121, GBM39 cells
Concentration:1 μM
Incubation Time:48 h
Result:Suppressed the expression of CD133, OLIG2, and SOX2 and cell proliferation marker KI67.
体内研究
(In Vivo)

CP681301 (1 mM; fed; 10 days) shows anti-tumor activity in 0- to 2-day-old adultDrosophila[1].

Animal Model:0- to 2-day-old adultDrosophila(brat-RNAi tumors)[1]
Dosage:1 mM
Administration:Fed, 10 days
Result:Reduced active phospho-dCdk5 (Y15) in tumor cells and the self-renewal properties of stem cells, increased the expression of neuronal marker ElaV in these cells and increased the median survival by 2.8-fold.
分子量

298.38

性状

Solid

Formula

C17H22N4O

CAS 号

865317-32-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL(335.14 mM)

*"≥" means soluble, but saturation unknown.

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM3.3514 mL16.7572 mL33.5143 mL
5 mM0.6703 mL3.3514 mL6.7029 mL
10 mM0.3351 mL1.6757 mL3.3514 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。