Mirin is a potentMre11-Rad50-Nbs1 (MRN) complexinhibitor. Mirin prevents MRN-dependent activation ofATM(IC₅₀=12 μM) without affectingATMprotein kinase activity, and it inhibits Mre11-associated exonuclease activity. Mirin abolishes the G2/M checkpoint and homology-dependent repair in mammalian cells. Mirin preventsATMactivation in response to DNA double-strand breaks (DSBs) and blocks homology-directed repair (HDR) in mammalian cells^[1].

Mirin inhibits H2AX phosphorylation with an IC₅₀of 66 μM. Mirin also inhibits the ATM-dependent phosphorylation of the downstream targets Nbs1 and Chk2 and the MRN-dependent autophosphorylation of ATM at Ser1981 in response to DSBs. Mirin induces a substantial G2 arrest at concentrations of 50 μM and 100 μM. Mirin (10-100 μM) inhibits homology-dependent DNA repair in TOSA4 cells^[1].
BRCA2-deficient cells also showed hypersensitivity to the Mre11 inhibitor Mirin^[2].

220.25

Solid

C₁₀H₈N₂O₂S

299953-00-7

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

DMSO : 83.33 mg/mL(378.34 mM;Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。