生物活性
Kaempferol 3-O-β-D-glucuronide is a conjugated kaempferol metabolite, which has anti-inflammatory effect. Kaempferol 3-O-β-D-glucuronide significantly inhibits various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4.
化学数据
| 分子量 | 462.36 |
| 分子式 | C21H18O12 |
| CAS号 | 22688-78-4 |
| 纯度 | >99% |
| 溶解性（25°C） | DMSO |
| 储存和运输条件 | 2-8°C, protect from light, dry, sealed
常温运输及临时存放 |
实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）
| 细胞实验 |
|---|
| 细胞系 | RAW 264.7 cells |
| 方法 | RAW 264.7 cells (2×105cells/well) were pretreated with K3G (5 and 10 μM) for 1 h and stimulated with LPS (1 μg/mL) for 24 h. Cells were then harvested. Cell pellet was solubilised in RIPA buffer (Sigma; R-0278) over ice for 40 min. The lysate was centrifuged at 14,000 g for 15 min at 4 °C. Supernatant was collected. Protein estimation was done by Bradford's method with BSA standard. Western blotting was performed according to the reported protocol. Detection of specific proteins was done by Immobilon Western (Chemiluminescent HRP substrate, Millipore) mediated chemiluminescence by ChemiDoc XRS+(Bio Rad). Densitometry of specific bands was done using Image Lab Software version 3 (Bio Rad). The effect on phosphorylation of NF-kB p65 was studied in a dose dependent manner in comparison to dexamethasone (0.5 μM). |
| 浓度 | 5 and 10 μM |
| 处理时间 | 1 h |
| 动物实验 |
|---|
| 动物模型 | Female Balb/C mice 10–12 weeks old and weight is 20–25 g |
| 配制 | dissolved in 100% DMSO and the working concentration of DMSO in animals is below 1% |
| 剂量 | 1 mg/kg, 2 mg/kg and 5 mg/kg |
| 给药处理 | drugged for 1 h |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.1628 mL | 10.8141 mL | 21.6282 mL |
| 5 mM | 0.4326 mL | 2.1628 mL | 4.3256 mL |
| 10 mM | 0.2163 mL | 1.0814 mL | 2.1628 mL |
m.cnreagent.com