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Coenzyme Q0
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Coenzyme Q0图片
CAS NO:605-94-7
包装与价格：
包装价格(元)
10 mM * 1 mL in DMSO电议
500mg电议
1 g电议
5 g电议

产品名称
CoQ0
产品介绍
Coenzyme Q0 (CoQ0) 是一种口服有效的醌类化合物，可以从Antrodia cinnamomea中得到。Coenzyme Q0 诱导细胞凋亡 (apoptosis) 和自噬 (autophagy)，抑制 HER-2/AKT/mTOR 信号通路来增强细胞凋亡和自噬机制。Coenzyme Q0 调节 NFκB/AP-1 的激活，并增强 Nrf2 的稳定，减轻炎症和氧化还原失衡。Coenzyme Q0 通过下调 MMP-9/NF-κB 和上调 HO-1 信号通路具有抗血管生成活性。
生物活性

Coenzyme Q0 (CoQ0) is a potent, oral active ubiquinone compound can be derived fromAntrodia cinnamomea. Coenzyme Q0 inducesapoptosisandautophagy, suppresses of HER-2/AKT/mTOR signaling to potentiate theapoptosisandautophagymechanisms. Coenzyme Q0 regulates NFκB/AP-1 activation and enhances Nrf2 stabilization in attenuation of inflammation and redox imbalance. Coenzyme Q0 has anti-angiogenic activity through downregulation of MMP-9/NF-κB and upregulation of HO-1 signaling[1][2][3].

体外研究
(In Vitro)

Coenzyme Q0 (0-40 μM; 24 h) and inhibits viability and growth of human ovarian carcinoma cells[1].
Coenzyme Q0 (CoQ0) (0-30 μM; 24 h; SKOV-3 cells) has anti-proliferative activity through induction of G2/M cell-cycle arrest and reduction of cell-cycle regulatory proteins[1].
Coenzyme Q0 (CoQ0) (0-30 μM; 0-30 min; SKOV-3 cells) increases intracellular ROS levels to promote SKOV-3 cell death[1].
Coenzyme Q0 (CoQ0) (0-30 μM; 24 h; SKOV-3 cells) induces autophagy by increase accumulation of LC3-II, GFP-LC3 puncta, AVOs formation and Beclin-1/Bcl-2 dysregulation[1].
Coenzyme Q0 (CoQ0) (0-30 μM; 24 h; SKOV-3 cells) induces apoptosis by mitochondrial (caspase-3, PARP and Bax/Bcl-2 dysregulation) and ER stress (caspase-12 and Hsp70) signals[1].
Coenzyme Q0 (CoQ0) (30 μM; 24 h; SKOV-3 cells) suppresses of HER-2/AKT/mTOR signaling to potentiate the apoptosis and autophagy mechanisms[1].
Coenzyme Q0 (CoQ0) (0-10 μM; 0.5-18 h; RAW264.7 cells) regulates NFκB/AP-1 activation and enhances Nrf2 stabilization[2].
Coenzyme Q0 (CoQ0) (5 μM; 0-12 h; EA.hy 926 cells) has anti-angiogenic activity in EA.hy 926 cells[3].

Cell Viability Assay[1]

Cell Line:SKOV-3, A2780 and A2870/CP70 cells
Concentration:0, 10, 20, 30 and 40 μM
Incubation Time:24 hours
Result:Decreased viability with the IC50values of 26.6 μM, 27.3 μM and 28.4 μM for SKOV-3, A2780 and A2870/CP70 cells, respectively.

Cell Cycle Analysis[1]

Cell Line:SKOV-3, A2780 and A2870/CP70 cells
Concentration:0, 10, 20 and 30 μM
Incubation Time:24 hours
Result:Arrested cell cycle at G2/M phase and reduced cell-cycle proteins in SKOV-3 cells.

Apoptosis Analysis[1]

Cell Line:SKOV-3, A2780 and A2870/CP70 cells
Concentration:0, 5, 15 and 30 μM
Incubation Time:24 hours
Result:Promoted the conversion of LC3–1 to LC3-II and increased the LC3-II accumulation. Increased Bax/Bcl-2 ratio in a dose-dependent manner.

Apoptosis Analysis[1]

Cell Line:SKOV-3 cells
Concentration:0, 10, 20 and 30 μM
Incubation Time:24 hours
Result:Had the percentage of early apoptotic cells are 25.1%, 34% and 36% for 10, 20 and 30 μM, respectively.

Western Blot Analysis[1]

Cell Line:SKOV-3 cells
Concentration:0, 5, 15 and 30 μM
Incubation Time:24 hours
Result:Activated of caspase-3 and cleavaged of PARP. Increased the expressions of caspase-12, HSP-70 and Bax in a dose-dependent manner, decreased the expressions of Bcl-2.

Western Blot Analysis[1]

Cell Line:SKOV-3 cells
Concentration:30 μM
Incubation Time:24 hours
Result:Decreased the phosphorylated HER-2 (Y1221) levels, p-AKT (Ser473) and p-mTOR (S2448) levels.

Western Blot Analysis[2]

Cell Line:RAW264.7 cells
Concentration:0, 2.5, 5 and 10 μM
Incubation Time:0.5-18 hours
Result:Inhibited iNOS/COX-2 protein expressions with reductions of NO, PGE2, TNF-α and IL-1β secretions.

Western Blot Analysis[3]

Cell Line:EA.hy 926 cells
Concentration:5 μM
Incubation Time:0, 1, 3, 6 and 12 hours
Result:Increased expressions of heme oxygenase-1 (HO-1) and γ-glutamylcysteine synthetase (γ-GCLC), inhibits protein expressions of matrix metalloproteinase-9 (MMP-9), reduces TNF-α-induced nuclear translocation and transcriptional activation of nuclear factor-κB (NF-κB).
体内研究
(In Vivo)

Coenzyme Q0 (CoQ0) (1.5 and 2.5 mg/kg; i.p.; once every four days, for 52 d) suppresses tumor growth in SKOV-3 xenografted nude mice[1].
Coenzyme Q0(CoQ0) (5 mg/kg; p.o.; for 4 h) has anti-inflammatory activities through Nrf2 activation and NFκB inhibition in liver and spleen of LPS-treated mice[2].

Animal Model:SKOV-3 xenografted nude mice[1]
Dosage:1.5 and 2.5 mg/kg
Administration:Intraperitoneal injection; Once every four days, for 52 days
Result:Inhibited the tumor growth at 1.5 and 2.5 mg/kg.
Animal Model:LPS-treated female FVB mice[2]
Dosage:5 mg/kg
Administration:Oral administration; for 4 hours
Result:Down-regulates inflammatory genes in liver and spleen tissues of LPS injected mice.
分子量

182.18

性状

Solid

Formula

C9H10O4

CAS 号

605-94-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL(274.45 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM5.4891 mL27.4454 mL54.8908 mL
5 mM1.0978 mL5.4891 mL10.9782 mL
10 mM0.5489 mL2.7445 mL5.4891 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 1.67 mg/mL (9.17 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (9.17 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 1.67 mg/mL (9.17 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (9.17 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在本网站选购。