Novobiocin Sodium

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Novobiocin Sodium

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1476-53-5

包装与价格：

包装	价格(元)
10 mM * 1 mL in DMSO	电议
100mg	电议
500mg	电议
1 g	电议
5 g	电议

产品名称

Albamycin sodium
Cathomycin sodium

产品介绍

Novobiocin (Albamycin) sodium 是一种有效且具有口服活性的抗生素。Novobiocin sodium 还是 DNA 促旋酶抑制剂和热休克蛋白 90 (Hsp90) 拮抗剂。Novobiocin sodium 具有研究高度耐药性肺炎球菌感染的潜力。Novobiocin Sodium 具有抗正痘病毒活性^{[ 4]}。

生物活性

Novobiocin (Albamycin) sodium is a potent and orally activeantibiotic. Novobiocin sodium also is aDNA gyraseinhibitor and aheat shock protein90 (Hsp90)antagonist. Novobiocin sodium has the potential for the research of highly beta-lactam-resistant pneumococcal infections. Novobiocin sodium shows anti-orthopoxvirus activity^[1]^[2]^[3]^[4].

IC₅₀& Target^[1]

β-lactam

HSP90

体外研究
(In Vitro)

Novobiocin sodium (1 mM) competitively inhibits ATP binding to gyrase B to interfere with nucleotide binding and interferes with the association of the co-chaperones Hsc70 and p23 with Hsp90^[1].
Novobiocin sodium (200 μM; 24 h) inhibits the rate of repair of both cis-DDP and BCNU induced DNA interstrand cross-links and with a corresponding decrease in the clonogenic survival of the human glioblastoma multiforme cells^[2].
Novobiocin sodium (0.3 mM; 48 hours) induces a caspase-3/7 enzyme–dependent apoptosis assays with an induction of approximately three- to fivefold of apoptotic cells in K562, HL60, Mutz-2^[5].

Western Blot Analysis^[2]

Cell Line:	K562, HL60, Mutz-2 cells
Concentration:	0.3 mM
Incubation Time:	48 hours
Result:	Decreased caspase-3/7 activity in K562, HL60, Mutz-2 cells.

体内研究
(In Vivo)

Novobiocin sodium (25, 50, 100, 200 mg/kg; s.c.; 4 times at 1, 5, 24 and 48 h after infection) shows anti-infection activity in mice infected with amoxicillin-resistantStreptococcus pneumoniae^[3].

Animal Model:	30 g adult female Swiss mice (sepsis induced by the penicillin-susceptible strain (AR33118))^[3]
Dosage:	25, 50, 100, 200 mg/kg
Administration:	S.c.; given at 1, 5, 24 and 48 h after infection
Result:	Showed anti-infection activity in mice infected with amoxicillin-resistantS. pneumoniae.

分子量

634.61

性状

Solid

Formula

C₃₁H₃₅N₂NaO₁₁

CAS 号

1476-53-5

中文名称

新生霉素钠

结构分类

Antibiotics
Other Antibiotics

来源

Streptomyces niveus

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 50 mg/mL(78.79 mM;Need ultrasonic)

DMSO : ≥ 30 mg/mL(47.27 mM)

*"≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.5758 mL	7.8789 mL	15.7577 mL
5 mM	0.3152 mL	1.5758 mL	3.1515 mL
10 mM	0.1576 mL	0.7879 mL	1.5758 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (157.58 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。