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4,5-Dicaffeoylquinic acid
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
4,5-Dicaffeoylquinic acid图片
CAS NO:57378-72-0
包装与价格:
包装价格(元)
10 mM * 1 mL in DMSO电议
5mg电议
10mg电议
50mg电议
100mg电议

产品名称
异绿原酸C
Isochlorogenic acid C
产品介绍
4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) 是一种抗氧化剂,可从Gynura divaricataLaggera alata中分离获得。4,5-Dicaffeoylquinic acid 能减少 2 型糖尿病小鼠胰岛细胞凋亡 (apoptosis),改善胰腺功能,对酵母 α-葡萄糖苷酶 (α-glucosidase) 有明显的抑制活性。4,5-Dicaffeoylquinic acid 通过阻滞细胞周期来抑制前列腺癌细胞增殖。4,5-Dicaffeoylquinic acid 还具有抗凋亡、抗损伤和抗乙型肝炎病毒 (HBV)的作用。
生物活性

4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) is an antioxidant, can be isolated fromGynura divaricataandLaggera alata. 4,5-Dicaffeoylquinic acid reduces islet cellapoptosisand improves pancreatic function in type 2 diabetic mice, and has obvious inhibitory activities against yeastα-glucosidase. 4,5-Dicaffeoylquinic acid inhibits prostatecancercells through cell cycle arrest. 4,5-Dicaffeoylquinic acid also has anti-apoptotic, anti-injury and anti-hepatitis B viruseffects[1][2][3].

IC50& Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

4,5-Dicaffeoylquinic acid (0.1~100 μM; 72 h) has dose-dependent inhibitory activity against DU-145 prostate cancer cells, and induces cell cycle arrest, also induces inactivation of Bcl-2[2].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 48 h) has anti-apoptotic effect in D-GalN-challenged HL-7702 hepatocytes, and improves significantly cell viability at concentrations of 10 to 100 μg/mL[3].
4,5-Dicaffeoylquinic acid (1~100 μg/mL; 4 days) inhibits significantly expressions of HBsAg and HBeAg, and produces the maximum inhibition rates of 86.93 and 59.79% at 100 μg/mL on expressions of HBsAg and HBeAg, respectively[3].

Cell Proliferation Assay[2]

Cell Line:DU-145 cells
Concentration:0.1~100 μM
Incubation Time:72 h
Result:Exhibits dose-dependent inhibitory activity against DU-145 prostate cancer cells with an IC50of 5 μM.

Cell Cycle Analysis[2]

Cell Line:DU-145 cells
Concentration:5 μM
Incubation Time:72 h
Result:Decreased in numbers in G0/G1 phase and increased in S phase.

Apoptosis Analysis[3]

Cell Line:HL-7702 hepatocytes (exposure to 60 mM D-GalN for 6h)
Concentration:1~100 μg/mL
Incubation Time:48 h
Result:Significantly reduced the caspase-3 and transforming growth factor β1 (TGFβ1) levels of the D-GalN-challenged hepatocytes at 10~100 μg/mL.

Cell Viability Assay[3]

Cell Line:HL-7702 hepatocytes (exposure to 80 mM D-GalN for 6h)
Concentration:1~100 μg/mL
Incubation Time:48 h
Result:Improved significantly cell viability at concentrations of 10 to 100 μg/mL, and produced a maximum protection rate of 47.28% at 100 μg/mL.
体内研究
(In Vivo)

4,5-Dicaffeoylquinic acid enhances the expression of GLUT2, GK, and PDX-1 protein, and effectively alleviate glycaemia and insulin resistance and enhance insulin sensitivity in type 2 diabetic mice[1].

分子量

516.45

性状

Solid

Formula

C25H24O12

CAS 号

57378-72-0

中文名称

异绿原酸C

结构分类
  • Phenols
  • Polyphenols
  • Ketones, Aldehydes, Acids
来源
  • Plants
  • Leguminosae
  • Bowdichia virgilioides
  • Plants
  • Compositae
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder-20°C3 years
4°C2 years
In solvent-80°C6 months
-20°C1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL(96.81 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.9363 mL9.6815 mL19.3630 mL
5 mM0.3873 mL1.9363 mL3.8726 mL
10 mM0.1936 mL0.9681 mL1.9363 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40%PEG300   5%Tween-80   45% saline

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH2O 中,得到澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20%SBE-β-CDin saline)

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90%corn oil

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在本网站选购。