4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) 是一种抗氧化剂,可从Gynura divaricata和Laggera alata中分离获得。4,5-Dicaffeoylquinic acid 能减少 2 型糖尿病小鼠胰岛细胞凋亡 (apoptosis),改善胰腺功能,对酵母 α-葡萄糖苷酶 (α-glucosidase) 有明显的抑制活性。4,5-Dicaffeoylquinic acid 通过阻滞细胞周期来抑制前列腺癌细胞增殖。4,5-Dicaffeoylquinic acid 还具有抗凋亡、抗损伤和抗乙型肝炎病毒 (HBV)的作用。
生物活性 | 4,5-Dicaffeoylquinic acid (Isochlorogenic acid C) is an antioxidant, can be isolated fromGynura divaricataandLaggera alata. 4,5-Dicaffeoylquinic acid reduces islet cellapoptosisand improves pancreatic function in type 2 diabetic mice, and has obvious inhibitory activities against yeastα-glucosidase. 4,5-Dicaffeoylquinic acid inhibits prostatecancercells through cell cycle arrest. 4,5-Dicaffeoylquinic acid also has anti-apoptotic, anti-injury and anti-hepatitis B viruseffects[1][2][3]. |
IC50& Target | Human Endogenous Metabolite |
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体外研究 (In Vitro) | 4,5-Dicaffeoylquinic acid (0.1~100 μM; 72 h) has dose-dependent inhibitory activity against DU-145 prostate cancer cells, and induces cell cycle arrest, also induces inactivation of Bcl-2[2]. 4,5-Dicaffeoylquinic acid (1~100 μg/mL; 48 h) has anti-apoptotic effect in D-GalN-challenged HL-7702 hepatocytes, and improves significantly cell viability at concentrations of 10 to 100 μg/mL[3]. 4,5-Dicaffeoylquinic acid (1~100 μg/mL; 4 days) inhibits significantly expressions of HBsAg and HBeAg, and produces the maximum inhibition rates of 86.93 and 59.79% at 100 μg/mL on expressions of HBsAg and HBeAg, respectively[3].
Cell Proliferation Assay[2] Cell Line: | DU-145 cells | Concentration: | 0.1~100 μM | Incubation Time: | 72 h | Result: | Exhibits dose-dependent inhibitory activity against DU-145 prostate cancer cells with an IC50of 5 μM. |
Cell Cycle Analysis[2] Cell Line: | DU-145 cells | Concentration: | 5 μM | Incubation Time: | 72 h | Result: | Decreased in numbers in G0/G1 phase and increased in S phase. |
Apoptosis Analysis[3] Cell Line: | HL-7702 hepatocytes (exposure to 60 mM D-GalN for 6h) | Concentration: | 1~100 μg/mL | Incubation Time: | 48 h | Result: | Significantly reduced the caspase-3 and transforming growth factor β1 (TGFβ1) levels of the D-GalN-challenged hepatocytes at 10~100 μg/mL. |
Cell Viability Assay[3] Cell Line: | HL-7702 hepatocytes (exposure to 80 mM D-GalN for 6h) | Concentration: | 1~100 μg/mL | Incubation Time: | 48 h | Result: | Improved significantly cell viability at concentrations of 10 to 100 μg/mL, and produced a maximum protection rate of 47.28% at 100 μg/mL. |
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体内研究 (In Vivo) | 4,5-Dicaffeoylquinic acid enhances the expression of GLUT2, GK, and PDX-1 protein, and effectively alleviate glycaemia and insulin resistance and enhance insulin sensitivity in type 2 diabetic mice[1].
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结构分类 | - Ketones, Aldehydes, Acids
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来源 | - Plants
- Leguminosae
- Bowdichia virgilioides
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Powder | -20°C | 3 years | | 4°C | 2 years | In solvent | -80°C | 6 months | | -20°C | 1 month |
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溶解性数据 | In Vitro: DMSO : 50 mg/mL(96.81 mM;Need ultrasonic) 配制储备液 1 mM | 1.9363 mL | 9.6815 mL | 19.3630 mL | 5 mM | 0.3873 mL | 1.9363 mL | 3.8726 mL | 10 mM | 0.1936 mL | 0.9681 mL | 1.9363 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用;
以下溶剂前显示的百 分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂: 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂: 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。 3. 请依序添加每种溶剂: 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (4.84 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。 *以上所有助溶剂都可在本网站选购。
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