Verrucarin J (Muconomycin B) 是Myrothecium真菌家族的代谢物。Verrucarin J 诱导活性氧 (ROS) 生成和癌细胞系凋亡 (apoptosis),例如 A549、HCT 116 和 SW-620 细胞。Verrucarin J 具有抗Candida albicans和Mucor miehei的活性。Verrucarin J 抑制沙粒病毒 Junin (JUNV) 产量,IC50为 1.2 ng/mL。
生物活性 | Verrucarin J (Muconomycin B) is a metabolite of theMyrotheciumfungus family. Verrucarin J generatesreactive oxygen species(ROS) and inducesapoptosisofcancercell lines, such as A549, HCT 116 and SW-620 cells. Verrucarin J shows activities againstCandida albicansandMucor miehei. Verrucarin J inhibitsarenavirusJunin (JUNV) yield with anIC50of 1.2 ng/mL[1][2][3][4][5]. |
体外研究 (In Vitro) | Verrucarin J (0, 5, 10, 20, 50 nM; 24 hours) induces the apoptosis of A549 cells[1]. Verrucarin J (0, 1, 2, 5, 10, 20, 50 nM; 24, 48, 72 hours) significantly inhibits cell proliferation of A549 and H1793 cells with IC50values of approximately 10 nM and 20 nM after 48 h of treatment, respectively[1]. Verrucarin J (0, 0.1, 0.2, 0.3, 0.4, 0.5 μM; 24 hours) has an IC50of 300 nM for HCT 116 and SW-620 cell proliferation[2]. Verrucarin J (0, 10, 20 nM, 48 hours) inhibits cancer stem cell (CSC) self-renewal pathways Wnt1/β-catenin and Notch1 and down-regulates the expression of key CSC specific genes (ALDH1, LGR5, OCT4 and CD133) of A549 cells[1]. Verrucarin J (compound 2; 50 μg/disk) shows noteworthy activities againstCandida albicansandMucor miehei[3]. Verrucarin J reduces JUNV yield more than 2 log units and has a similar effect against the arenavirus Tacaribe[4]. Verrucarin J reduces the cell viability of Vero cells with a cytotoxic concentration 50%
(CC50) of 8.2 ng/mL[4].
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体内研究 (In Vivo) | Verrucarin J (0.5 mg/kg; i.p. for 4 weeks) suppresses AKT-induced tumor growth in a xenograft model[2]. Verrucarin J (0.1, 0.5, 2.0 mg/kg; i.p. for three weeks) is a highly potent anticancer drug and suppresses tumor growth and metastasis[5].
Animal Model: | 6-8 weeks old BALB/c athymic nude mice (nu/nu) with pCMV/HCT 116 and AKT/HCT 116 xenografts[2] | Dosage: | 0.5 mg/kg body weight | Administration: | i.p. for 4 weeks | Result: | Reduced the expression of prosurvival markers pAKT, Notch1, p65, and Ki67 in all tumors. |
Animal Model: | Female nude nu/nu (5 to 6 weeks old) mice with A2780 xenografts[5] | Dosage: | 0.1, 0.5, 2.0 mg/kg (vehicle: 10% DMSO, 90% glyceryl trioctanoate) | Administration: | i.p. for three weeks after 10 days of injection of A2780 cells | Result: | Reduced tumor weight (32% lower compared to control), and reduced visible metastasis in 0.1 mg/kg. Showed a significant reduction in visible peritoneal tumors (61% lower compared to control group) and highly reduced visible metastasis in 0.5 mg/kg. Reduced ovarian tumor weight by 71% compared to vehicle in 0.5 mg/kg. In lethal dose 2 mg/kg, mice sick with a swollen belly, body fluid and subsequently died within 3 treatments. |
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来源 | Myrothecium fungus family, Stachybotrys chartarum |
运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |