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G-418 disulfate
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
G-418 disulfate图片
CAS NO:108321-42-2
包装与价格:
包装价格(元)
10 mM * 1 mL in Water电议
500mg电议
1 g电议
5 g电议
10 g电议
50 g电议

产品名称
Geneticin sulfate
Antibiotic G-418 sulfate
产品介绍
G-418 disulfate (Geneticin sulfate) 是一种氨基糖苷类抗生素,抑制真核生物和原核生物的蛋白质合成。G-418 disulfate 是一种常用的真核细胞选择性药剂。
生物活性

G-418 disulfate (Geneticin sulfate), is anaminoglycosideantibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1].

IC50& Target

Aminoglycoside

 

体外研究
(In Vitro)

G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2].
The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml[4].

分子量

692.71

性状

Solid

Formula

C20H44N4O18S2

CAS 号

108321-42-2

中文名称

遗传霉素硫酸盐

结构分类
  • Antibiotics
  • Other Antibiotics
来源

Micromonospora rhodorangea

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

溶解性数据
In Vitro: 

H2O : 125 mg/mL(180.45 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.4436 mL7.2180 mL14.4361 mL
5 mM0.2887 mL1.4436 mL2.8872 mL
10 mM0.1444 mL0.7218 mL1.4436 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80℃, 6 months; -20℃, 1 month (stored under nitrogen, away from moisture)。-80℃ 储存时,请在 6 个月内使用,-20℃ 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 50 mg/mL (72.18 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在本网站选购。