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Cy5 hydrazide(non-sulfonated)
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Cy5 hydrazide(non-sulfonated)图片
包装与价格：
包装价格(元)
5mg电议
25mg电议

产品介绍

化学性质

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt569.61
FormulaC32H42Cl2N4O
Solubility≥48 mg/mL in DMSO
Canonical SMILESCC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(N[NH3+])=O)C4=CC=CC=C4C3(C)C
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Cy5 hydrazide is a reactive dye which reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. The labeling regent is used for the labeling of aldehydes and ketones. The dye examples are proteins subjected to oxidative stress, glycoproteins pre-activated by periodate oxidation, and oligonucleotides with aldehyde moieties. Cy5 hydrazide could replace carbonyl-reactive Alexa Fluor 647 and DyLight 649 dyes. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In HL-60 cells, Protein carbonylation induced by Hydrogen peroxide (H2O2) was quantitatively analyzed by using fluorescent dyes of Cy5-hydrazide dye, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and fluorescence determination [1].

Reference:
[1] Yoshimura, T. ; Harashima, M.; Kurogi, K.; Suiko, M.; Liu, M.-C.; Sakakibara, Y. A novel procedure for the assessment of the antioxidant capacity of food components. Analytical Biochemistry, 2016, 507, 7–12.

Spectral properties

最大激发光波 (nm)646最大发射光波 (nm)662
消光系数 (M-1cm-1)250000量子产量0.2
CF2600.03CF2800.04