PMSF

立即咨询

PMSF

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

329-98-6

包装与价格：

包装	价格(元)
10mM (in 1mL DMSO)	电议
10g	电议
100g	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	174.2
Cas No.	329-98-6
Formula	C₇H₇FO₂S
Solubility	insoluble in H2O; ≥17.4 mg/mL in DMSO; ≥28.3 mg/mL in EtOH
Chemical Name	phenylmethanesulfonyl fluoride
Canonical SMILES	C1=CC=C(C=C1)CS(=O)(=O)F
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

PMSF(苯甲基磺酰氟)是丝氨酸蛋白酶的一个不可逆抑制剂，与迟发性神经病的发展有关。它在许多类型的组织中对胞内修复和再生起作用。PMSF是一个长期作用的神经病靶标酯酶(NTE)抑制剂。NTE是一个保护剂，与迟发性多发性神经病(OPIDP)的假定靶点的抑制作用有关。PMSF可以将NTE抑制作用增加90%以上。PMSF还可作为γ-谷酰基转肽酶的一个活性位点定向试剂。

参考文献：
[1]. Thomas Baker, Herbert E. Lowndes, Martin K. Johnson, Irene C. Sandborg. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology. 1980; 46(3-4): 305 – 311.
[2]. Marcello Lotti, Stefano Caroldi, Eugenio Capodicasa, Angelo Moretto. Promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. Toxicology and Applied Pharmacology. 1991; 108(2): 234 – 241.
[3]. Masayasu Inoue, Seikoh Horiuchi, Yoshimasa Morino. Inactivation of γ-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. Biochemical and Biophysical Research Communications. 1978; 82(4): 1183 – 1188.

试验操作

Cell experiment:[1]
Cell lines	Longitudinal smooth muscle of guinea pig ileum
Reaction Conditions	2 mM PMSF for 30 min incubation
Applications	PMSF inhibited carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15% ~ 19%. The inhibition effect of PMSF on phosphoinositide turnover was due to one or more steps following phosphoinositide breakdown.
Animal experiment:[2]
Animal models	Cats
Dosage form	30 mg/kg Administered intraperitoneally (i.p.)
Applications	Pretreatment of cats with PMSF (30 mg/kg i.p.) 24 h before the diisopropylfluorophosphate (DFP) injection protected the cats from the delayed neuropathy. No clinical neurotoxic signs were observed at 21 days after DFP exposure. The stimulus-bound repetitive capacity of soleus α-motor nerve terminals was not lost at this time and its incidence was much greater than that which occurred in cats not pretreated with PMSF.
Note	The technical data provided above is for reference only.
	References: 1. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984, 5(3): 191-203. 2. Baker T, Lowndes HE, Johnson MK, et al. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology, 1980, 46(3-4): 305-311.