Sodium butyrate

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Sodium butyrate

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

156-54-7

包装与价格：

包装	价格(元)
10mM (in 1mL H2O)	电议
1g	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	110.09
Cas No.	156-54-7
Formula	C₄H₇NaO₂
Solubility	insoluble in DMSO; ≥4 mg/mL in H2O; ≥5.87 mg/mL in EtOH
Chemical Name	sodium;butanoate
Canonical SMILES	CCCC(=O)[O-].[Na+]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Sodium butyrate is a kind of histone deacetylases (HDACs) inhibitors studied clinically, which works on histones and transcription factors to modulate transcription [1].

Interaction of HDACs and histone acetyltransferases determines histone acetylation, which affects the final transcription process. The transcription dysregulation results in functional and degenerative changes [2].

In the adenoma RG/C2 cell line, sodium butyrate reduced the number of attached cells to 50% of that of the control group, and the number of floating cells is increased induced by cell apoptosis. Compare with RG/C2, AA/CI showed more sensitivity to sodium butyrate [3].

In the experiments of the R6/2 cell line transgenic Huntingtin disease (HD) mouse model, seven different intraperitoneal dosings of sodium butyrate were administrated daily. The survival time, weight and athletic ability were improved together, and the neuropathologic sequelae were delayed, too. Besides, sodium butyrate increased the acetylation level of histone and specificity protein-1 and prevent the neurotoxicity inducing by 3-nitropropionic acid [1].

Reference:

[1] Ferrante R J, Kubilus J K, Lee J, et al. Histone deacetylase inhibition by sodium butyrate chemotherapy ameliorates the neurodegenerative phenotype in Huntington's disease mice [J]. Journal of Neuroscience, 2003, 23(28): 9418-9427.

[2] Cha J H. Transcriptional dysregulation in Huntington's disease [J]. Trends in Neurosciences, 2000, 23(9): 387-392.

[3] Hague A, Manning A M, Hanlon K A, et al. Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway: implications for the possible role of dietary fibre in the prevention of large-bowel cancer [J]. International Journal of Cancer, 1993, 55(3): 498-505.

试验操作

细胞实验 [1]:
细胞系	2种源于腺瘤的细胞系（AA/Cl和RG/C2）
制备方法	在DMSO中的溶解度有限。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。
反应条件	1 ~ 4 mM；4天
实验结果	在RG/C2细胞中，Sodium Butyrate在浓度为2 mM及以上时使贴壁细胞产量降至对照组的约50%，同时显著增加游离细胞的比例。实验表明，游离细胞百分比的增加归因于细胞凋亡的诱导，而不仅仅是由于坏死增加。与RG/C2细胞相比，AA/Cl细胞对Sodium Butyrate更为敏感。
动物实验 [2]:
动物模型	亨廷顿氏病 (HD) R6/2转基因小鼠模型
给药剂量	100、200、400、 600、1200、5000和10,000 mg/kg；腹腔注射；每天1次
实验结果	在HD R6/2转基因小鼠模型中，Sodium Butyrate呈剂量依赖性地显著延长小鼠存活期、改善体重和运动能力，并延迟神经病理后遗症。此外，Sodium Butyrate增加组蛋白以及特异性蛋白-1的乙酰化水平，并防止3-硝基丙酸诱导的神经毒性。
注意事项	请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同，但仍处于实验系统误差的允许范围内。
References: [1]. Hague A1, Manning AM, Hanlon KA, Huschtscha LI, Hart D, Paraskeva C. Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway: implications for the possible role of dietary fibre in the prevention of large-bowel cancer. Int J Cancer. 1993 Sep 30;55(3):498-505. [2]. Ferrante RJ1, Kubilus JK, Lee J, Ryu H, Beesen A, Zucker B, Smith K, Kowall NW, Ratan RR, Luthi-Carter R, Hersch SM. Histone deacetylase inhibition by sodium butyrate chemotherapy ameliorates the neurodegenerative phenotype in Huntington's disease mice. J Neurosci. 2003 Oct 15;23(28):9418-27.