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Oxytocin Acetate
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Oxytocin Acetate图片
CAS NO:6233-83-6
包装与价格：
包装价格(元)
10mM (in 1mL DMSO)电议
5mg电议
10mg电议
50mg电议

产品介绍

化学性质

StorageStore at -20°C
M.Wt1067.25
Cas No.6233-83-6
FormulaC45H70N12O14S2
Synonymsα-Hypophamine acetate; Oxytocic hormone acetate
Solubility≥182.8 mg/mL in DMSO; ≥14.93 mg/mL in EtOH; ≥44.1 mg/mL in H2O
Chemical Namerac-(R)-N-((R)-1-((2-amino-2-oxoethyl)amino)-4-methyl-1-oxopentan-2-yl)-1-((4S,7R,10R,13R,16R,19S)-19-amino-7-(2-amino-2-oxoethyl)-10-(3-amino-3-oxopropyl)-13-((R)-sec-butyl)-16-(4-hydroxybenzyl)-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentaazacyclo
Canonical SMILESO=C([C@H]1N(C([C@H](CSSC[C@@H]2N)NC([C@H](CC(N)=O)NC([C@H](CCC(N)=O)NC([C@H]([C@@H](C)CC)NC([C@H](CC(C=C3)=CC=C3O)NC2=O)=O)=O)=O)=O)=O)CCC1)N[C@H](C(NCC(N)=O)=O)CC(C)C.OC(C)=O
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资料参考

Oxytocin is a positive allosteric modulator of μ opioid receptor (MOR) signaling [1].

Oxytocin, a 9-amine neuropeptide synthesized in the paraventricular nucleus (PVN) and the supraoptic nucleus of the hypothalamus, is secreted from the posterior pituitary into the systemic circulation, playing an essential role in mammalian labor, lactation, maternal bonding, and social affiliation. Oxytocin has been reported to exert an analgesic effect as well [1].

In hMOR-expressing HEK293 cells, Oxytocin alone showed no direct agonistic effect on human MOR at concentration up to 10-5M, but treatment with 10-6M Oxytocin markedly enhanced the MOR signaling stimulated by 10-6M endomorphin-1, β-endorphin, morphine, fentanyl, as well as DAMGO. Moreover, in the competitive receptor-binding assay, 10-6M Oxytocin did not alter the affinity of endomorphin-1 or morphine for MOR [1].

Intracerebroventricular Oxytocin administration (0.2 μg) robustly increased medial nucleus accumbens shell (NAcSh) neuron mean firing rate in rats, but did not do so after rats were repeatedly treated with morphine [2].

References:

[1]. Meguro Y, Miyano K, Hirayama S, et al. Neuropeptide oxytocin enhances μ opioid receptor signaling as a positive allosteric modulator. Journal of Pharmacological Sciences, 2018, 137(1): 67-75.

[2]. Moaddab M, Hyland B I, Brown C H. Oxytocin excites nucleus accumbens shell neurons in vivo. Molecular and Cellular Neuroscience, 2015, 68: 323-330.

试验操作

Cell experiment:[1]

Cell lines

Human Embryonic Kidney 293 (HEK293) cells stably expressing human μ opioid receptor (hMOR)

Reaction Conditions

10-10~ 10-5M oxytocin

Applications

In hMOR-expressing HEK293 cells, oxytocin alone showed no direct agonistic effect on hMOR at concentration up to 10-5M, but treatment with 10-6M oxytocin markedly enhanced the MOR signaling stimulated by 10-6M endomorphin-1, β-endorphin, morphine, fentanyl, as well as DAMGO. Neuropeptide oxytocin could enhance MOR signaling as a positive allosteric modulator.

Animal experiment:[2]

Animal models

Adult male Wistar rats aged 6 ~ 8 weeks and weighing 250 ~ 300 g

Dosage form

0.2 μg

Intracerebroventricular administration

Applications

Intracerebroventricular oxytocin administration (0.2 μg) robustly increased medial nucleus accumbens shell (NAcSh) neuron mean firing rate in rats, but did not do so after rats were repeatedly treated with morphine.

Note

The technical data provided above is for reference only.

References:

1. Meguro Y, Miyano K, Hirayama S, et al. Neuropeptide oxytocin enhances μ opioid receptor signaling as a positive allosteric modulator. Journal of Pharmacological Sciences, 2018, 137(1): 67-75.

2. Moaddab M, Hyland B I, Brown C H. Oxytocin excites nucleus accumbens shell neurons in vivo. Molecular and Cellular Neuroscience, 2015, 68: 323-330.