Latrunculin A

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Latrunculin A

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

76343-93-6

包装与价格：

包装	价格(元)
50ug (solution)	电议
100ug (solution)	电议

产品介绍

化学性质

Physical Appearance	A solution in ethanol
Storage	Store at -20°C
M.Wt	421.55
Cas No.	76343-93-6
Formula	C₂₂H₃₁NO₅S
Solubility	Limited solubility, soluble in DMSO
Chemical Name	(R)-4-((1R,4Z,8E,10Z,12S,15R,17R)-17-hydroxy-5,12-dimethyl-3-oxo-2,16-dioxabicyclo[13.3.1]nonadeca-4,8,10-trien-17-yl)thiazolidin-2-one
Canonical SMILES	O[C@@](C1)([C@H]2NC(SC2)=O)O[C@H](CC[C@@H](/C=C\C=C\CC/C(C)=C\3)C)C[C@H]1OC3=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Latrunculin A is a bioactive 2-thiazolidinone macrolide derived from the red sea spongeLatrunculia magnifica, that sequesters G-actin and prevents F-actin assembly. It binds monomeric actin with 1:1 stoichiometry and can be used to block actin polymerization both in vitro and cells[1,2].

Latrunculin A (1-10 μM) causes tumor cytoskeleton disaggregation in ten minutes[3]. Treatment of cells with latrunculin A at 10 μM overnight strongly inhibits actin synthesis [4].

References：

[1]. Yarmola E G, Somasundaram T, Boring T A, et al. Actin-latrunculin A structure and function. The Journal of Biological Chemisty, 2000, 275(36): 28120-28127.

[2]. Loubéry S, Wilhelm C, Hurbain I, et al. Different microtubule motors move early and late endocytic compartments. Traffic, 2008, 9(4): 492-509.

[3]. Hayot C, Debeir O, Van Ham P, et al. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicology and Applied Pharmacology, 2006, 211(1): 30-40.

[4]. Lyubimova A, Bershadsky A D, Ben-Ze'ev A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry, 1999, 76(1): 1-12.

试验操作

Cell experiment:[1]
Cell lines	SV-80 cells
Reaction Conditions	10 μM latrunculin A
Applications	Latrunculin A treatment (2 h) induced a retraction of the cell body, leaving behind long cellular processes, while stress fibers were not apparent in such cells. Analysis of the distribution of actin between a Triton X-100-soluble and an insoluble fraction showed that while in control cells about 50% of actin was in the Triton X-100-soluble fraction, in latrunculin A-treated cells (12 h) nearly all actin became Triton X-100 soluble.
Note	The technical data provided above is for reference only.
	References: 1. Lyubimova A, Bershadsky AD, Ben-Ze'ev A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry, 1999, 76(1): 1-12.