Phorbol 12,13-dibutyrate

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CAS NO:	37558-16-0
包装:	1mg
市场价:	1008元

产品介绍

化学性质

Physical Appearance	White lyophilised solid
Storage	Store at -20°C
M.Wt	504.61
Cas No.	37558-16-0
Formula	C₂₈H₄₀O₈
Solubility	<25.23mg/ml in DMSO
Chemical Name	(1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-1H-cyclopropa[3,4]benzo[1,2-e]azulene-9,9a-diyl dibutyrate
Canonical SMILES	O[C@@]1([C@H](C=C(C)C2=O)[C@]32O)[C@@H](C=C(CO)C3)[C@H](C4(C)C)[C@]4([C@@H]([C@H]1C)OC(CCC)=O)OC(CCC)=O
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Phorbol 12,13-dibutyrate, a phorbol ester, is an activator of PKC, including the conventional calcium-dependent subtypes (α and γ), the nonconventional calcium-independent isoforms (δ, ε, η), as well as the atypical calcium-independent and DAG or phorbol ester-unresponsive subtype (ζ). PKC plays an important role in the regulation of a variety of cellular processes such as exocytosis, gene expression, cell proliferation and differentiation, as well as tumor promotion, thereby representing the major target for tumor-promoting phorbol esters and chemically unrelated tumor promoters. Phorbol esters are able to replace the endogenous activator DAG in the stimulation of PKC, and participate in the multistage process of tumor formation and progression.

References:

1. Geiges D, Meyer T, Marte B, et al. Activation of protein kinase C subtypes alpha, gamma, delta, epsilon, zeta, and eta by tumor-promoting and nontumor-promoting agents. Biochemical Pharmacology, 1997, 53(6): 865-875.

2. Middleton JP, Khan WA, Collinsworth G, et al. Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. Journal of Biological Chemistry, 1993, 268(21): 15958-15964.

3. Scribner JD, Boutwell RK. Inflammation and tumor promotion: selective protein induction in mouse skin by tumor promoters. European Journal of Cancer, 1972, 8(6): 617-621.

试验操作

Cell experiment:[2]
Cell lines	OK and LLC-PK₁cells expressing detectable levels of PKCα and PKCζ
Reaction Conditions	1 mM phorbol 12,13-dibutyrate for 10 min incubation
Applications	Phorbol 12,13-dibutyrate activated PKC, and inhibited Na/K-ATPase transport activity in OK cells, but not in LLC-PK₁cells.
Animal experiment:[3]
Animal models	Female CD-1 mice, 8 weeks of age
Dosage form	Female CD-1 mice, 8 weeks of age Topical administration
Applications	Phorbol 12,13-dibutyrate induced papilloma formation in mice with a total dose of 9.7 μmol required to produce papillomas in 50% of survivors after eight weeks. Phorbol 12,13-dibutyrate also induced inflammation in 50% of mice when administered topically to the ear at a dose of 0.067 μmol/ear.
Note	The technical data provided above is for reference only.
	References: 1. Geiges D, Meyer T, Marte B, et al. Activation of protein kinase C subtypes alpha, gamma, delta, epsilon, zeta, and eta by tumor-promoting and nontumor-promoting agents. Biochemical Pharmacology, 1997, 53(6): 865-875. 2. Middleton JP, Khan WA, Collinsworth G, et al. Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. Journal of Biological Chemistry, 1993, 268(21): 15958-15964. 3. Scribner JD, Boutwell RK. Inflammation and tumor promotion: selective protein induction in mouse skin by tumor promoters. European Journal of Cancer, 1972, 8(6): 617-621.