特性

o Amine-reaction—Reacting with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides.
o Labeling antibody—This kit can label antibodies to facilitate immobilization, purification or detection.
o Labeling protein—This kit can label proteins to facilitate immobilization, purification or detection.
o Labeling Cell surface molecules—This kit can label the cell surface proteins because the negatively charged reagent does not permeate cell membranes.
o Irreversible-Permanent amide bonds are formed; Spacer arm cannot be cleaved.
o Very short arm—Spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.
o Solubility increased—Sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds.

NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling. NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

常见问题解答

1）Q：货号K1001-K1006的生物素化试剂盒中，生物素产品与哪些基团反应进行生物素化？

A：该系列试剂盒中生物素试剂（如Sulfo-NHS-LC-Biotin）上的NHS酯与伯胺反应。在蛋白质和多肽中，伯胺基团一般存在于赖氨酸残基的氨基末端和ε胺（侧链）上。

2）Q：带有磺酸基（Sulfo-）的Biotin与不带的Biotin有什么不同？

A：磺酸基（Sulfo-）Biotin可增加水溶性，常用于细胞表面标记；non-sulfonated Biotin需用DMSO或DMF等有机溶剂溶解，可透过细胞膜，常用于标记细胞内带有伯胺（NH2-）的蛋白。

3）Q：K1001-K1006试剂盒中Biotin产品有什么不同？

A：第一，带磺酸基（Sulfo-）的Biotin与不带Sulfo-的Biotin膜渗透性、水溶性不同；第二，(Sulfo) -NHS-Biotin试剂是一种短链生物素化试剂， (Sulfo) -NHS-LC-Biotin试剂是一种中等长度的生物素化试剂，(Sulfo) -NHS-SS-Biotin是一种间隔臂包含可裂解的二硫键的生物素化试剂。

4）HABA分析常见问题