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Sulforaphane
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Sulforaphane图片
CAS NO:4478-93-7
包装与价格：
包装价格(元)
10mg电议
25mg电议
50mg电议

产品介绍

化学性质

StorageStore at -20°C, 避光保存
M.Wt177.3
Cas No.4478-93-7
FormulaC6H11NOS2
SynonymsSFN
Solubility≥51.6 mg/mL in H2O; ≥58.2 mg/mL in EtOH; ≥67.6 mg/mL in DMSO
Chemical Name1-isothiocyanato-4-(methylsulfinyl)-butane
Canonical SMILESCS(CCCCN=C=S)=O
运输条件蓝冰运输或根据您的需求运输。
一般建议为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Sulforaphane is an inducer of chemopreventative enzymes via Keap1-Nrf2 signaling [1]. Sulforaphane is present naturally in widely consumed vegetables and has a particularly high concentration in broc-coli [1].

The Keap1-Nrf2 pathway is the major regulator of cytoprotective responses to oxidative and electrophilic stress. Nrf2 triggers the signaling pathways to prevent cancer initiation and progression in normal and premalignant tissues. In fully malignant cells, Nrf2 activity provides growth advantage by increasing cancer chemoresistance and enhancing tumor cell growth [2].

In human colon carcinoma cells (HT29) cells, sulforaphane at 15 μM inhibited cell growth and induced cell death. Sulforaphane induced a cell cycle arrest in a dose-dependent manner, followed by cell death. Sulforaphane induced cell cycle arrested in G2 -M Phase, which was correlated with increased expression of cyclins A and B1. Sulforaphane increased expression of the proapoptotic protein bax, the release of cytochrome c from the mitochondria to the cytosol, and the proteolytic cleavage of poly(ADP-ribose) polymerase [1].

References:
[1] Gamet-Payrastre L, Li P, Lumeau S, et al.  Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells[J]. Cancer research, 2000, 60(5): 1426-1433.
[2] Kansanen E, Kuosmanen S M, Leinonen H, et al.  The Keap1-Nrf2 pathway: mechanisms of activation and dysregulation in cancer[J]. Redox biology, 2013, 1(1): 45-49.

试验操作

Cell experiment:[1]

Cell lines

HT29 human colon cancer cells

Reaction Conditions

0 ~ 30 μM sulforaphane for 48 h incubation

Applications

Sulforaphane resulted in a net decrease in the total number of cells and an accumulation of cells floating in the culture medium. The inhibition of growth and the effect on the adherence were already important at 15 μM. Sulforaphane at 15 μM was able to inhibit cell growth and induce cell death. Cell mortality was already 75% at 24 h and almost total at 96 h.

Animal experiment:[2]

Animal models

Female Sprague-Dawley rats, 40 days of age

Dosage form

75 or 150 μmol

Once daily by gavage for 5 days

Applications

When sulforaphane was administered by gavage (75 or 150 μmol per day for 5 days) around the time of exposure to the 9,10-dimethyl-1,2-benzanthracene carcinogen, the incidence, multiplicity, and weight of mammary tumors were significantly reduced, and their development was delayed. Sulforaphane was able to protect against carcinogenesis.

Note

The technical data provided above is for reference only.

References:

1. Gamet-Payrastre L, Li P, Lumeau S, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Research, 2000, 60(5): 1426-1433.

2. Zhang Y, Kensler TW, Cho CG, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(8): 3147-3150.