Folinic acid

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Folinic acid

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

58-05-9

包装与价格：

包装	价格(元)
10mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	473.44
Cas No.	58-05-9
Formula	C₂₀H₂₃N₇O₇
Solubility	≥7.62 mg/mL in DMSO with gentle warming; insoluble in H2O; insoluble in EtOH
Chemical Name	(2S)-2-(4-(((5-formyl-4-hydroxy-2-imino-1,2,5,6,7,8-hexahydropteridin-6-yl)methyl)amino)benzamido)pentanedioic acid
Canonical SMILES	N=C1NC(NCC(N2C=O)([H])CNC3=CC=C(C(N[C@@](C(O)=O)([H])CCC(O)=O)=O)C=C3)=C2C(O)=N1
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Folinic acid, clinically known as leucovorin, is a ubiquitous member of biological folate pools, usually accounting for 10 ~ 20% of total cellular folates. Folinic acid can reduce methotrexate (MTX)-induced cytogenetic damage, and appears to be an anticlastogenic agent. Folinic acid serves as a rescue agent used alone or in combination with 5-fluorouracil to decrease MTX-induced toxicity.

References:

1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228.

2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.

试验操作

Cell experiment:[1]
Cell lines	V79 cells
Reaction Conditions	5 or 50 μg/ml folinic acid for 40 h incubation
Applications	MTX alone induced a concentration-related increase in the percentages of micronucleated binucleated cells (MNBN) and aberrant cells (Abs). The addition of folinic acid at 50 μg/ml significantly reduced MNBN by 40 ~ 68% and Abs by 36 ~ 77%. Inhibition was also seen at 5 μg/ml folinic acid (12 to 54% for MNBN and 20 to 61% for Abs).
Animal experiment:[2]
Animal models	Balb/c young growing male mice, 11.9 ± 0.25 g
Dosage form	7.0 mg/kg Injected intraperitoneally every second day for 3 weeks
Applications	Chronic administration of MTX induced suppression of skeletal growth in mice. Folinic acid treatment following MTX administration appeared to reverse this growth inhibition.
Note	The technical data provided above is for reference only.
	References: 1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228. 2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.