Pleuromutilin

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Pleuromutilin

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

125-65-5

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
50mg	电议

产品介绍

化学性质

Physical Appearance	A crystalline solid
Storage	Store at 4°C
M.Wt	378.5
Cas No.	125-65-5
Formula	C₂₂H₃₄O₅
Synonyms	Antibiotic A 40104C,Drosophilin B,NSC 121145
Solubility	≥90.6 mg/mL in DMSO; ≥103.4 mg/mL in EtOH; insoluble in H2O
Chemical Name	2-hydroxy-acetic acid-(3aS,4R,5S,6S,8R,9R,9aR,10R)-6-ethenyldecahydro-5-hydroxy-4,6,9,10-tetramethyl-1-oxo-3a,9-propano-3aH-cyclopentacycloocten-8-yl ester
Canonical SMILES	O=C1CCC2(CC[C@H]3C)[C@@H](C)[C@H](O)[C@@](C=C)(C)C[C@@H](OC(CO)=O)C3(C)[C@@]21[H]
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

Pleuromutilin is a diterpene antibiotic derived from the basidiomycete fungus, Clitopilus passeckerianus [1][2].

Pleuromutilin is a biologically active compound produced by Clitopilus passeckerianus, Drosophila subatrata, Clitopilus scyphoides (Pleurotus mutilus), and several other species of the genus Clitopilus. Pleuromutilin is a tricyclic diterpene that against gram-positive bacteria, such as Staphylococcus aureus, Streptococcus haemolyticus, and Bacillus subtilis. tiamulin and valnemulin, the derivatives of pleuromutilin, are used in veterinary medicine to treat swine dysentery and enzootic pneumonia. A novel semisynthetic pleuromutilin, retapamulin, is active against Staphylococcus aureus and Streptococcus pyogenes and is the first pleuromutilin derivative formulated as a topical antibacterial agent for treating human skin infections [1].

Pleuromutilin is a diterpene antibiotic that inhibits bacterial protein synthesis by binding to bacterial ribosomes in the peptidyl transferase center of the 50S subunit and inhibiting peptide bond formation [2].

References:
[1]. Kilaru S, Collins CM, Hartley AJ, et al. Establishing molecular tools for genetic manipulation of the pleuromutilin-producing fungus Clitopilus passeckerianus. Appl Environ Microbiol. 2009 Nov;75(22):7196-204.
[2]. Long KS, Hansen LH, Jakobsen L, et al. Interaction of pleuromutilin derivatives with the ribosomal peptidyl transferase center. Antimicrob Agents Chemother. 2006 Apr;50(4):1458-62.

试验操作

细胞实验 [1]：
细胞系	大肠杆菌菌株
溶解方法	在DMSO中的溶解度≤ 25mg/ml。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。
反应条件	200～250 μg/ml，>400 μg/ml
应用	相对于亲本菌株，L3突变菌株对tiamulin, pleuromutilin, and SB-264128敏感性降低。相反，在亲本和L3突变菌株之间没有观察到对valnemulin的敏感性差异。这很重要，因为在实验室筛选出的短螺旋体突变体和猪痢疾短螺旋体野外分离株中，在核糖体蛋白L3的相应位置发生突变，导致其对tiamulin的敏感性降低。
References: [1]. Long KS, Hansen LH, Jakobsen L, Vester B. Interaction of pleuromutilin derivatives with the ribosomal peptidyl transferase center. Antimicrob Agents Chemother. 2006 Apr;50(4):1458-62. doi: 10.1128/AAC.50.4.1458-1462.2006. PMID: 16569865; PMCID: PMC1426994.